Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-06
1999-09-07
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435473, 4353201, 536 231, 536 241, C12Q 168, C12N 1574, C12N 1563, C07H 2104
Patent
active
059486228
ABSTRACT:
A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
REFERENCES:
Ahmed, Asad, "Use of Transposon-Promoted Deletions in DNA Sequence Analysis", Letter to Editor in J.Mol. Biol., 178:941-948 (1984).
Benjamin, Howard W., "Excisionof Tn10 from the donor site during transposition occurs by flush double-strand cleavages at the transposon termini," Proc. Natl. Acad. Sci. USA, 89:4648-4652 (May 1992).
Craigie, Robert, et al., "A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: Protein and DNA substrate requirements," Proc. Natl. Acad. Sci. USA, 82:7570-7574 (Nov. 1985).
de la Cruz, Norberto, B., et al., "Characterization of the TN5 Transposase and Inhibitor Proteins: a Model for the Inhibition of Transposition," Journal of Bacterioilogy, 175(21):6932-6938 (Nov. 1993).
Devine et al., "Efficient Integration of Artificial Transposons into Plasmid Target in vitro: a Useful Tool for DNA Mapping, Sequencing and Genetic Analysis", Nucleic Acid Research, 22 No. 18:3765-3772 (1994).
Devine et al., "A Transposon-base Strategy for Sequencing Repetitive DNA in Eukaryotic Genomes", In Press, Genome Research (1998).
Hattori et al., "A Novel Method for Making Nested Deletions and Its Application for Sequencing of a 300 kb Region of Human APP Locus", Nucleic Acids Research, 25 No. 9: 1802-1808 (1997).
Henikoff, Steven, "Unidirectional Digestion with Exonuclease III Creates Targeted Breakpoints for DNA Sequencing", Gene, 28:351-359 (1984).
Jilk, Ross A., et al., "The Organization of the Outside End of Transposon Tn5," Journal of Bacteriology, 178(6):1671-1679 (Mar. 1996).
Jilk et al., "Implications of Tn5-Associated Adjacent Deletions", J. of Bacteriology, 175: 1264-1271 (1993).
Krishnan et al., "Construction of a Genomic DNA `Feature Map` by Sequencing from Nested Deletions: Application to the HLA Class I Region", Nucleic Acids Research, 23 No. 1: 117-122 (1995).
Lavoie, B.D., et al., "Transposition of Phage Mu DNA," Dept. of Biochemistry, U. of Western Ontario.
Lu et al., "Characterization of the Interaction Between the Tn7 Transposase (TnsA+B) and the Transposase Regulator TnsC", Keystone Symposia: Transposition and Site-Specific Recombination, Santa Fe (1997).
Mizuuchi, Kiyoshi, "In Vitro Transposition of Bacteriophage Mu: A Biochemical Approach to a Novel Replication Reaction," Cell, 35:785-794 (1983).
Morisata, Donald, et al., "TN10 Transposition and Circle Formation In Vitro," Cell, 51:101-111 (1987).
Morita et al., "Nested Deletions from a Fixed Site as an Aid to Nucleotide Sequencing: An in vitro System Using Tn3 Transposase", DNA Research, 3:431-433 (1996).
Pues et al., "Construction of a Deletion Library Using a Mixture of 5'-Truncated Primers for Inverse PCR (IPCR)", Nucleic Acids Research, 25(6): 1303-1304 (1997).
Sakai, Janice, et al., "Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in TN10 transposition," The EMBO Journal, 14(17):4374-4383 (1995).
Strathmann et al., "Transposon-facilitated DNA Sequencing", Proc. Natl. Acad. Sci., 88: 1247-1250 (1991).
Tomacsanyi et al., "Intramolecular Transposition by a Synthetic IS50 (Tn5) Derivative", J. Bacteriology, 172(11):6348-6354 (1990).
Wang et al., "Inversions and Deletions Generated by a Mini-.gamma..delta. (Tn1000) Transposon", J. of Bacteriology, 176(5): 6348-6354 (1990).
Wang et al., "pDUAL: A Transposon-Based Cosmid Cloning Vector for Generating Nested Deletions and DNA Sequencing Templated in vivo", Proc. Natl. Acad. Sci., 90: 7874-7878 (1993).
Weinreich, Michael D., "A Functional Analysis of the TN5 Transposase: Identification of Domains Required for DNA Binding and Multimerization," J. Mol. Biol., 241:166-177 (1994).
Yohda et al., "Solid-Phase Nested Deletion: A New Subcloning-less Method for Generating Nested Deletions", DNA Research, 2: 175-181 (1995).
Zhu and Marshall, "Rapid Construction of Nested Deletions of Recombinant Plasmid DNA for Dideoxy Sequencing", BioTechniques, 18(2): 222-224 (1995).
Delong et al., "Trans-acting Transposase Mutant from Tn5" Proc. Natl. Acad. Sci. USA 88(14):6072-6076 (1991).
Johnson et al., "DNA Sequences at the Ends of Transposon Tn5 Required for Transposition", Nature 304(21):280-282 (1983).
Wiegand et al., "Characterization of Two Hypertransposing Tn5 Mutants", J. Bacteriol. 174(4):1229-1239 (1992).
Wiegand et al., "Transposase Mutants That Increase the Transposition Frequency of Tn5", PhD Thesis (Abstract) University of Wisconsin-Madison (1993).
Zhou et al., "Three Types of Novel Mutations in the NH-2-Terminus of Tn5 Transposase: Structure-function of Transposase", Keystone Symposium on Transposition and Site-Specific Recombination: Mechanism and Biology (Abstract) Jan. 1994. J. of Cell Biochem. Suppl. O(18B) (1994).
Goryshin Igor Yu
Reznikoff William S.
York Dona L.
Zhou Hong
Degen Nancy
Sandals William
Wisconsin Alumni Research Foundation
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