System for growing multi-layered cell cultures

Details System for growing multi-layered cell cultures System for growing multi-layered cell cultures

1995-06-30

1997-02-11

Redding, David A.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

4352971, 4352972, 4352975, 435399, 210615, C12N 500, C12M 300

Patent

active

056020287

ABSTRACT:
Method and apparatus for growing cell cultures consisting of a plurality of layers includes a chamber for submerging cells in a medium while supporting and binding them to a semi-permeable membrane. A stirrer is positioned within the chamber and continuously stirs the medium to constantly circulate it past the exposed surface of a semi-permeable membrane on one side of the cell culture and along the exposed surface of the cell culture growing on the membrane and remote from the membrane to transfer nutrients to both surfaces of the cell either directly or through the membrane without disrupting the cell culture on the membrane. Preferably, multi-layered cell cultures so grown while still adhered to the membrane and growing is then mounted in a partition separating a pair of chambers to provide substantially the sole passage from one chamber to the other by flow through the multi-layer culture while in the living state whereby the rate of penetration or degree of penetration of a culture being tested by the material being tested may be measured by measuring the quantity of material in one chamber versus the quantity of that material in the other chamber.

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"Three-Dimensional Histoculture: Origins and Applications in Cancer Research" by Hoffman Cancer Cells 1991 vol. 3 No. 3.
"Multicellular Membranes: An In Vitro Model for Assessing Transport in the Extravascular Compartment of a Tumor"-Wilson et al.
15th Annual Meeting North American Hyperthermia Society Apr. 1-6 1995.
Multilayers of Cells Growing on a Permeable Support: An In Vitro Tumour Model. Studies with Radiation and Tirapazamine by Andrew I. Minchinton et al. Abstract 15 Jul. 1994-(inventor disclosure).

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