System for efficient isolation of genes using probes

Details System for efficient isolation of genes using probes System for efficient isolation of genes using probes

1985-10-10

1988-11-29

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 31, 935 79, 935 80, 4351723, 435320, C12Q 168, C12N 1500, C12N 100

Patent

active

047881356

ABSTRACT:
Systems are provided for the dual purpose of cloning genes using antibodies as probes and isolating unknown proteins encoded by cloned DNA. The method employs a vector derived from phage which is used in combination with a high frequency lysogenic host. The vector is further characterized by having an inducible promoter regulating expression of a gene into which the foreign DNA may be introduced to produce a fused protein, and controlled induction of the prophage with rapid increase in copy number and high level transcription of foreign DNA. The technique is exemplified with a specific lambda phage construct in conjunction with the .beta.-galactosidase structural gene lacZ.

REFERENCES:
patent: 4466917 (1984-08-01), Nussenzweig et al.
Skalka and Shapiro, Gene (1976), 1:65-79.
Sanzey et al., Proc. Natl. Acad. Sci. USA, (1976), 73:3394-3397.
Erlich et al., Cell, (1978), 13:681-689.
Broome and Gilbert, Proc. Natl. Acad. Sci. USA, (1978), 75:2746-2749.
Clarke et al., Methods Enzymol., (1979), 68:436-442.
Kemp and Cowman, Proc. Natl. Acad. Sci. USA, (1981), 78:4520-4524.
Leder et al, Science, vol. 196, pp. 175-177, Apr. 1977.
Williams and Blattner, (1980), Bacteriophage Lambda Vectors for DNA Cloning in Genetic Engineering, 2:201.

Affiliated with

Also associated with

Rating

System for efficient isolation of genes using probes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with System for efficient isolation of genes using probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for efficient isolation of genes using probes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-363208