System for detection of nitrosylated proteins

Details System for detection of nitrosylated proteins System for detection of nitrosylated proteins

2008-09-02

2008-09-02

Wallenhorst, Maureen M. (Department: 1797)

Chemistry: analytical and immunological testing

Nitrogen containing

Oxides of nitrogen

C436S063000, C436S086000, C436S164000, C436S172000, C436S106000, C422S067000, C422S082050, C422S082080

Reexamination Certificate

active

10319457

ABSTRACT:
The present invention describes a novel, simplified method for detecting and monitoring whether the presence of nitrosylated proteins, such as S-nitrosoproteins, in a biological sample using fluorescence detection. The present invention further describes a method which can both quantify and identify the nature of nitrosylated proteins, which method is useful for monitoring both normal and disease states, in the development and screening of potential therapeutic drug species.

REFERENCES:
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Itoh et al., “Determination and Bioimaging Method for Nitric Oxide in Biological Specimens by Diaminofluorescein Fluorometry,” Analytical Biochemistry, 287:203-209 (2000).
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Kojima et al., “Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia,” Journal of Neurochemistry, 76:1404-1410 (2001).

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