System for detection of nitrosylated proteins

Chemistry: analytical and immunological testing – Nitrogen containing – Oxides of nitrogen

Reexamination Certificate

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C436S063000, C436S086000, C436S164000, C436S172000, C436S106000, C422S067000, C422S082050, C422S082080

Reexamination Certificate

active

10319457

ABSTRACT:
The present invention describes a novel, simplified method for detecting and monitoring whether the presence of nitrosylated proteins, such as S-nitrosoproteins, in a biological sample using fluorescence detection. The present invention further describes a method which can both quantify and identify the nature of nitrosylated proteins, which method is useful for monitoring both normal and disease states, in the development and screening of potential therapeutic drug species.

REFERENCES:
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patent: 5891735 (1999-04-01), Stamler et al.
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Beckman et al., “Methods in Nitric Oxide Research,” Feelisch and Stainler, Wiley, Chichester, U.K. (1996).
Fang et al., “Reductive Assays for S-nitrosothiols: Implications for measurements in biological systems,” Bioche. Biophys. Res. Commun. 252:535-40 (1998).
Mannick et al., “Fas-induced capsase denitrosylation,” Science 284:65 (1999).
Wang et al., “Inhibition of papain by S-nitrosothiols. Formation of mixed disulfides,” J. Biol. Chem. 275(27):20467-73 (2000).
Itoh et al., “Determination and Bioimaging Method for Nitric Oxide in Biological Specimens by Diaminofluorescein Fluorometry,” Analytical Biochemistry, 287:203-209 (2000).
King et al., “Assessment of S-nitrosothiols on diaminofluorescein gels.” Analytical Biochemistry, 346:69-76 (2005).
Kojima et al., “Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia,” Journal of Neurochemistry, 76:1404-1410 (2001).

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