Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-07-19
1999-10-12
Myers, Carla J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 911, 435810, 435 915, 536 2431, 536 2432, 536 2433, C12P 1934, C12Q 168, C07H 2104
Patent
active
059654090
ABSTRACT:
A system for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence that is substantially identical to the sequence of a mRNA having a known sequence. The first primer is used as a primer for reverse transcription of the MRNA and the resultant cDNA is amplified with a polymerase using both the first and second primers as a primer set.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5104792 (1992-04-01), Silver et al.
patent: 5262311 (1993-11-01), Pardee et al.
Chelly et al., "Transcription of the dystrophin gene in human muscle and non-muscle tissues", Nature, vol. 333, pp. 857-860, Jun. 1988.
Cohen, "Cloning of Protein-Serine/Threonine Phosphatases", 1991, Methods in Enzymology, vol. 201, pp. 398-408.
Freifelder, "Molecular Biology", pp. 402-404, 1983, Jones and Bartlett Publishers, Inc.
Fritz et al., "A novel 3' extension technique using random primers in RNA-PCR", Nucleic Acids Research, No. 13, Jul. 11, 1991.
Frohman et al., "Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer", Proc. Natl. Acad. Sci. U.S.A., vol. 85, No. 3, pp. 8998-9002, Dec. 1988.
Innis et al., "Competitive PCR for Quantitation of mRNA", pp. 60-69, 1990.
Khan et al., "Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers", Nucleic Acids Research, vol. 19, No. 7, p. 1715, 1991.
Kozak et al., "An analysis of vertebrate mRNA sequences: intimations of translational control", Journal of Cell Biology, vol. 115, No. 4, pp. 887-903, Nov. 1991.
Lee et al., "Positive selection of candidate tumor-suppressor genes by subtractive hybridization", Proc. Nat. Acad. Sci. U.S.A., vol. 88, pp. 2825-2829, Apr. 1991.
Liang et al., "Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction", Science, vol. 257, pp. 967-971, Aug. 14, 1992.
Liang et al., "Differential display of cloning of messenger RNAs from human breast cancer versus mammary epithelial cells", Cancer Research, vol. 51:6966-6968, Dec. 15, 1992.
Moore et al., Nuc. Acids Res., 18(7):1921, 1990.
Sambrook et al., Molecular cloning: a laboratory manual, Second Edition, Cold Spring Harbor Press, pp. 8.6-8.35, 1989.
Sargent et al., "Isolation of differentially expressed genes", Methods in Enzymology, vol. 152, pp. 423-433, 1987.
Schaefer et al., "Exclusive expression of Epstein-Barr virus nuclear antigen 1 in Burkitt lymphoma arises from a third promoter, distinct from the promoters used in latently infected lymphocytes", Aug. 1991, Proc. Natl. Acad. Sci, USA, vol. 88, pp. 6550-6554.
Smith et al., "Complexity and sequence identification of 24 rat V.beta. genes", Jul. 1, 1991, The Journal of Immunology, vol. 147, pp. 375-379.
Tse et al., "Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase", Gene, vol. 88, pp. 293-296, 1990.
Walker et al., "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system", Proc. Natl. Acad. Sci. U.S.A., vol. 89, pp. 392-396, Jan. 1992.
Welsh et al., "Fingerprinting genomes using PCR with arbitrary primers", Nucleic Acids Research, vol. 18, No. 24, pp. 7213-7218, 1990.
Wilks, "Cloning Members of Protein-Tyrosine Kinase Family Using Polymerase Chain Reaction", Methods in Enzymology, vol. 200, pp. 533-547, 1991.
Williams et al., "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, vol. 18, No. 22, pp. 6531-6535, 1990.
Xiao et al., "Characterization of a full-length cDNA which codes for the human spermidine/spermine N.sup.1 -Acetyltransferase", Aug. 30, 1991, vol. 179, No. 1, pp. 407-415.
Zelent et al., "Differentially expressed isoforms of the mouse retinoic acid receptor .beta. are generated by usage of two promoters and alternative splicing", 1991, The EMBO Journal, vol. 10, No,. 1, pp. 71-81.
Moore et al., Nuc. Acids Res. 18(7): 1921, 1990.
Welsh et al., Nuc. Acids Res. 18(24): 7213, 1990.
Khan et al., Nuc Acids. Res. 19(7): 1715, 1991.
Liang Peng
Pardee Arthur B.
Dana-Farber Cancer Institute
Myers Carla J.
LandOfFree
System for comparing levels or amounts of mRNAs does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System for comparing levels or amounts of mRNAs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for comparing levels or amounts of mRNAs will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-651390