System for cell-based screening

Details System for cell-based screening System for cell-based screening

2005-06-07

2005-06-07

Le, Long (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S007200, C435S007210, C435S029000, C435S040500, C435S040510, C435S288300, C436S063000, C436S172000, C436S501000, C436S800000, C436S807000, C436S809000, C250S201300, C348S040000, C348S345000, C356S300000, C356S319000, C356S326000, C356S328000, C382S128000, C382S129000, C382S133000, C382S173000, C382S254000, C382S255000, C382S286000, C382S291000

Reexamination Certificate

active

06902883

ABSTRACT:
The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.

REFERENCES:
patent: 4344816 (1982-08-01), Craighead et al.
patent: 4647531 (1987-03-01), Kamentsky
patent: 4673988 (1987-06-01), Jansson et al.
patent: 4762701 (1988-08-01), Horan et al.
patent: 4783401 (1988-11-01), Horan et al.
patent: 4859584 (1989-08-01), Horan et al.
patent: 4982739 (1991-01-01), Hemstreet et al.
patent: 5031797 (1991-07-01), Boris et al.
patent: 5072382 (1991-12-01), Kamentsky
patent: 5096807 (1992-03-01), Leaback
patent: 5107422 (1992-04-01), Kamentsky
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5233369 (1993-08-01), Carlotta et al.
patent: 5313264 (1994-05-01), Ivarsson et al.
patent: 5324591 (1994-06-01), Georger et al.
patent: 5326691 (1994-07-01), Hozier
patent: 5355215 (1994-10-01), Schroeder et al.
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5401629 (1995-03-01), Harpold et al.
patent: 5436128 (1995-07-01), Harpold et al.
patent: 5486855 (1996-01-01), Carlotta et al.
patent: 5491084 (1996-02-01), Chalfie et al.
patent: 5500071 (1996-03-01), Kaltenbach
patent: 5502467 (1996-03-01), Hoisington et al.
patent: 5527673 (1996-06-01), Reinhartz et al.
patent: 5548661 (1996-08-01), Price et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5567294 (1996-10-01), Dovichi et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5581487 (1996-12-01), Kelly et al.
patent: 5585069 (1996-12-01), Zanzucchi et al.
patent: 5670113 (1997-09-01), Akong et al.
patent: 5732150 (1998-03-01), Zhou et al.
patent: 5784162 (1998-07-01), Cabib et al.
patent: 5790710 (1998-08-01), Price et al.
patent: 5857786 (1999-01-01), Randolph
patent: 5869238 (1999-02-01), Morrison
patent: 5885840 (1999-03-01), Kamentsky
patent: 5919646 (1999-07-01), Okun et al.
patent: 5989835 (1999-11-01), Dunlay et al.
patent: 6103479 (2000-08-01), Taylor
patent: 6210910 (2001-04-01), Walt et al.
patent: 6238869 (2001-05-01), Kris et al.
patent: 6388788 (2002-05-01), Harris et al.
patent: 6573039 (2003-06-01), Dunlay et al.
patent: 6620591 (2003-09-01), Dunlay et al.
patent: 6671624 (2003-12-01), Dunlay et al.
patent: S61-31282 (1986-02-01), None
patent: H1-165958 (1989-06-01), None
patent: 4(1992)-69776 (1992-03-01), None
patent: 5-501151 (1993-03-01), None
patent: WO 87/02802 (1987-05-01), None
patent: WO 95/07463 (1995-03-01), None
patent: WO 95/21191 (1995-08-01), None
patent: WO 96/09598 (1996-03-01), None
patent: WO 96/23898 (1996-08-01), None
patent: WO 96/27675 (1996-09-01), None
Taylor et al. American Scientist. vol. 80, pp. 322-335 (1992).
Aplin and Hughes, (1997), Anal. Biochem., 113: pp. 144-148.
Bailey, et al., (1993), Nature, 366: pp. 44-48.
Barak et al., (1997),J. Biol. Chem, 272(44): 27497-27500.
Barber et al., (1996),Neuroscience Letters, 207:17-20.
Beggs (1997),J. of Biomolec. Screening, 2(2):71-78.
Bell, Jr., et al., (1987), J. Histochem. And Cytochem., 35:1375-1380.
Bhatia, et al., (1993), Analytical Biochemistry, 208: pp. 197-205.
Brejc, et al., (1997), Proc. Natl. Acad. Sci., 94: pp. 2306-2311.
Bright et al., (1987),J. Cell Biol., 104:1019-1033.
Bright et al., (1989),Methods in Cell Biology, 30:157-192.
Bright et al., (1989),J. Cell. Physiol., 141:410-419.
Bright et al., (1996),Cytometry, 24:226-233.
Brinkley, (1992), Bioconjugate Chem., 3: pp. 2-13.
Bulinski et al., (1997), J. Cell Science, 110: pp. 3055-3064.
Calvert, et al., (1994), Journal of Vacuum Science and Technology B12: pp. 3884-3997.
Calvert, et al., (1995), In Thin Films, vol. 20; Organic Thin Films and Surfaces: Directions for the Nineties, A Ulman, Ed. Academic Press, Boston, pp. 109-141.
Chalfie et al., (1994),Science, 263:802-805.
Channavajjala, et al., (1997), J. Cell Sci., 110: pp. 249-256.
Chen, et al., (1997), Biophysical Journal, “Changes in the Spectral Properties of a Plasma Membrane Lipid Analog During the First Seconds of Endocytosis in Living Cells”, vol. 72, pp. 37-50.
Cheng, et al., (1996), Nature Biotechnology. 14: pp. 606-609.
Chrisey, et al., (1994), Proceedings, Materials Research Society, 330: pp. 179-184.
Chrisey, et al., (1996), Nucleic Acids Research, 24: pp. 3031-3039.
Chrisey, et al., (1996), Nucleic Acids Research, 24: pp. 3040-3047.
Clarke and McNeil, (1992), J. Cell Science, 102: pp. 533-541.
Clarke et al., (1994), BioTechniques, 17: pp. 1118-1125.
Cohen, (1997),Biochemical J., 326:1-16.
Craighead, et al., (1980), Appl. Phys. Lett., 37: pp. 653-655.
Craighead, et al., (1982), J. Vac. Sci. Technology., 20: pp. 316-319.
Cubitt et al., (1995),Trends in Biochemical Science, 20:448-455.
Daaka et al., (1998),J. Biol. Chem., 273(2):685-688.
Davis et al., (1995),Dev. Biology, 170:726-729.
DeBiasio et al., (1996),Mol. Biol. Cell, 7:1259-1282.
Denk et al., (1990),Science, 248:73-76.
Deprez et al., (1997),J. Biol. Chem., 272(28):17269-17275.
Dulcey, et al., (1991), Science, 252: pp. 551-554.
Bulcey, et al., (1996), Langmuir, 12: pp. 1638-1650.
Ehrig, et al., (1995), FEBS Letter, 367: 163-166.
Ellenberg et al., (1997),J. Cell Biol., 138(6):1193-1206.
Farkas et al., (1993),Annu. Rev. Physiol., 55:785-817.
Federov et al., (1994),J. Mol. Biol., 241:480-482.
Firestone et al., (1991),Cytometry, 12:195-206.
Frisch, et al., (1996), Bioconjugate Chem., 7: pp. 180-186.
Gerrittsen et al., (1997),J. of Fluorescence, 7(1):11-15.
Giuliano et al., (1995),Curr. Op. Cell Biol., 7:4-12.
Giuliano et al., (1995),Methods in Neuroscience, 27:1-16.
Giuliano et al., (1987),Anal. Biochem., 167:362-371.
Giuliano et al., (1990), Optical Microscopy for Biology, pp. 543-557.
Giuliano et al., (1995),Annu. Rev. of Biophysics and Biomolecular Structure, 24:405-434.
Giuliano,(1996),Cell Motil. Cytoskel., 35:237-353.
Go et al., (1997),Analytical Biochemistry, 247:210-215.
Goldmacher, et al., (1992), Bioconjugate Chem., 3: pp. 104-107.
Goldman et al., (1995),Experimental Cell Research, 221:311-319.
Gonzales et al., (1995), Biophysics J., 69: pp. 1272-1280.
Gonzales et al., (1987),Digital Image Processing, pp. 391-448.
Gough et al., (1993),J. Cell Biol., 121(5):1095-1107.
Grabarek and Gergely, (1990), Anal. Biochem, 185: pp. 131-135.
Graham et al., (1973),Virology, 52:456-467.
Gratton et al., (1994),Proc. of the Microscopical Society of America, pp. 154-155.
Groen et al., (1985),Cytometry, 6:81-91.
Hahn et al., (1992),Nature, 359:736-738.
Hahn et al., (1993),Fluorescent and Luminescent Probes for Biological Activity, W.T. Mason, (ed.), pp. 349-359, Academic Press, San Diego.
Harms et al., (1984),Cytometry, 5:236-243.
Harootunian et al., (1993),Mol. Biol. of the Cell, 4:993-1002.
Haselhoff, et al., (1997), Proc. Natl. Acad. Sci., 94: pp. 2122-2127.
Haugland, Fluorescent Tracers of cell morphology and fluid flow, in Handbook of Fluorescent Probes and Research Chemicals, 6thedition, ed. By Spence, Molecular Probes, Inc. Eugene OR, pp. 325-331, (1996).
Heim and Tsien (1996),Curr. Biol., 6:178-182.
Htun et al., (1996),Proc. Natl. Acad. Sci., 93:4845-4850.
Hu et al., (1995),FEBS Letters, 369:331-334.
Johnson et al., (1996),Cell, 85:149-158.
Johnson et al., (1985), J. Electron Microscopy Tech., 2: pp. 129-138.
Kaether et al., (1995),FEBS Letters, 369:267-271.
Kahl, et al., (1997), J. Biomol. Screening, 2: pp. 33-40.
Kapur, et al., (1996), Journal of Biomedical Materials Research, 33: pp. 205-216.
Keb

Affiliated with

Also associated with

Rating

System for cell-based screening does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with System for cell-based screening, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for cell-based screening will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3466127