Chemistry: molecular biology and microbiology – Apparatus – Including condition or time responsive control means
Patent
1994-02-18
1997-08-12
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Apparatus
Including condition or time responsive control means
435 6, 435 911, 435 912, 4352872, 4352851, 422 50, 422 62, 236 1C, 236 15RG, 236 91R, 236 97, 935 76, 935 77, 935 87, 935 88, C12M 302, C12M 100, G01N 2100, C12N 1500
Patent
active
056564937
ABSTRACT:
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
REFERENCES:
patent: 3311303 (1967-03-01), Noyes
patent: 3392914 (1968-07-01), Nienstaedt
patent: 3856471 (1974-12-01), Winitz
patent: 3912913 (1975-10-01), Bunting
patent: 3983363 (1976-09-01), Alter
patent: 4008048 (1977-02-01), Hellemans
patent: 4206872 (1980-06-01), Levine
patent: 4312835 (1982-01-01), Zoltan et al.
patent: 4335620 (1982-06-01), Adams
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4362699 (1982-12-01), Verlander et al.
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4404845 (1983-09-01), Schrenker
patent: 4474015 (1984-10-01), Christmas et al.
patent: 4478094 (1984-10-01), Salomaa et al.
patent: 4483823 (1984-11-01), Umetsu et al.
patent: 4504733 (1985-03-01), Walsh
patent: 4517160 (1985-05-01), Galle et al.
patent: 4518700 (1985-05-01), Stephens
patent: 4534941 (1985-08-01), Stephens et al.
patent: 4544436 (1985-10-01), Chlosta et al.
patent: 4554839 (1985-11-01), Hewett et al.
patent: 4555486 (1985-11-01), Bahl et al.
patent: 4582788 (1986-04-01), Erlich
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4598049 (1986-07-01), Zelinka
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4685081 (1987-08-01), Richman
patent: 4708886 (1987-11-01), Nelson
patent: 4711851 (1987-12-01), McNamara
patent: 4737462 (1988-04-01), Mark et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4853332 (1989-08-01), Mark et al.
patent: 4858155 (1989-08-01), Okawa et al.
patent: 4865186 (1989-09-01), Coy et al.
patent: 4865986 (1989-09-01), Coy et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4933146 (1990-06-01), Meyer et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4981801 (1991-01-01), Suzuki et al.
patent: 5008182 (1991-04-01), Sninsky et al.
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5075216 (1991-12-01), Innis et al.
patent: 5333675 (1994-08-01), Mullis et al.
"PC Application Ideas" Instruments and Control Systems (Oct. 1980).
Techne TP-16 Operating Manual May 1984.
Techne TP-16 Operating Manual Dec. 1984.
Smith, M., Appl. of Synthetic Oligodeoxyribonucleotides . . . pub. in Nucleic Acids Synthesis: Applications to Molecule Biology and Genetic Engineering . . . Nucleic Acids Research--Nucleic Acids Symposium Series No. 7, pp. 387-396 (1980).
Koster, H., Introduction, pub. in Nucleic Acids Synthesis: Applications to Molecular Biology and Genetic Engineering . . . Nucleic Acids Research--Nucleic Acids Symposium Series No. 7, pp. 1-4 (1980).
Agarwal et al., Nature 277, 27 (1970).
Kleppe et al., Studies on Polynucleotides . . . , J. Molecular Biology, 56, 1971, pp. 341-361.
Panet et al., Studies on Polynucleotides . . . , J. Biological Chemistry, 242(16), 1974, pp. 5213-5221.
Khorana, Research Proposal Submitted to the Division of Biological Sciences, National Science Foundation re: NSF Grant, Prop.No. GB-36881X, Grant Prop.No. PCM73-06757, sub. Oct. 11, 1972, funded for the period Feb. 1, 1973 through Jan. 31, 1978.
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 1982.
Wallace et al., The use of synthetic oligonucleotides as hybridization probes . . . , Nucleic Acids Res., 9:4, pp. 879-894 (1981).
Suggs et al., Use of synthetic oligonucleotides as hybridization probes . . . PNAS USA, 78:11, pp. 6613-6617, (1981).
Suggs et al., Use of synethetic oligodeoxyribo-nucleotides, Developmental Biology Using Purified Genes, pp. 683-693, Academic Press, 1981.
Goeddel et al., Human leukocyte interferon produced by E. coli is biologically active, Nature, 287, pp. 411-416 (1980).
Wallace et al., Directed deletion of a yeast transfer RNA intervening sequence, Science, 209, pp. 1395-1400 (1980).
Wallace et al., A set of synthetic oligodeoxy-ribonucleotide primers for DNA sequencing in the plasmid vector pBR322, Gene, 16, pp. 21-26 (1981).
Hong, Sequencing of large double-stranded DNA using the dideoxy sequencing technique, Bioscience Reports, 2, pp. 907-912 (198).
Smith et al., Sequence of the gene for Iso-1-Cytochrome c in Saccharomyces cerevisiae, Cell, 16, 753-761 (1979).
Hong, A method for sequencing single-stranded cloned DNA in both directions, Bioscience Reports, 1, pp. 243-252 (1981).
Sanger et al., Nucleotide sequence of Bacteriophage .lambda. DNA, J. Molecular Biology, 162, pp. 729-773 (1982).
Zagursky et al., Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA, Gene Anal. Techn., 2, pp. 89-94, (1985).
Itakura et al., Synthesis and use of synthetic oligonucleotides, Ann. Rev. Biochem, 53, pp. 323-356, (1984).
Yansura et al., Studies on gene control regions IX. The effect of hypoxanthine-substituted lac operators on the lac operator-lac repressor interaction, J. Mol. Biol., 133, pp. 117-135 (1979).
Goeddel et al., Studies on gene control regions VI. The 5-methyl of thymine, a lac repressor recognition site, Nucleic Acids Research, 4:9, pp. 3039-3054 (1977).
Gergen et al., Filter replicas and permanent collections of recombinant DNA plasmids, Nucleic Acids Research, 7:8, pp. 2115-2136 (1979).
Rossi et al., An alternate method for synthesis of double-stranded DNA segments, J. Biol. Chem., 257:16, pp. 9226-9229 (1982).
Szostak et al., Hybridization with synthetic oligonucleotides, Methods in Enzymology, 68, pp. 419-428 (1979).
Zoller et al., Laboratory methods: oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single stranded DNA template, DNA, 3:6, pp. 479-488 (1984).
Woo, A sensitive and rapid method for recombinant phage screening, Methods in Enzymology, 68, pp. 389-395 (1979).
Stetler et al., Isolation of a cDNA clone for the human HLA-DR antigen .alpha. chain by using a synthetic oligonucleotide as a hybridization probe, PNAS USA, 79, pp. 5966-5970 (1982).
Cavalieri et al., DNA polymerase: evidence for multiple molecular species, PNAS USA, 59, pp. 951-958 (1968).
Yoshida et al., Multiple Molecular Species of Escherichia coli DNA polymerase, PNAS USA, 68:1, pp. 200-204 (1971).
Goulian et al., Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage .phi.X174 DNA, PNAS USA, 58, pp. 2321-2328 (1967).
Maniatis et al., Chain Length Determination of Small Double Stranded and Single Stranded DNA Molecules by Polyacrylamide Gel Electrophoresis, Biochemistry, 14: 17, pp. 3787-3794 (1975).
Sanger et al., Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage F1 DNA, PNAS USA, 70:4, pp. 1209-1213 (1973).
Deininger, Approaches to Rapid DNA Sequence Analysis, Anal. Biochemistry, 135, 247-263 (1983).
Tecam Dry Heat Baths, Techne Catalog 7051081, 1986.
Techne (OG-1 Black Digestor), Catalog 7051091, 1986.
Techne (Dri-Block 08-3), Catalog 7051101, 1986.
Histomat advertisement, R. Jung GmbH, Oct., 1980.
Techne Brochure for Temperature programmer TP-16, Dec., 1984.
P. S. Martin, et al., J. Parent, Sci. Tech., p. 63.
Techne Ad for Dri Block PHC-1.
Techne TP-16 Temperature Programmer Advertisement.
Biores b.v. Bioexcellence TAQ-Polymerase and Ampliclone Kit Ad.
Techne PHC-2 Ad.
Techne PCH-2 Temperature Cycler Ad.
Techne Flow Coolers FC-200 and FC200 and Dip Cooler RU-200.
Techne Tempunit or Tempette Immersion Circulators Ad.
Brookfield Test Chamber.
Cole-Parmer Instr. Co. 1985-86 Catalog.
IEEE Transactions on Biomedical Engineering, vol. BME-29, No. 8, Aug. 1982, pp. 557-568 V.J. Anselmo, et al. "Programmable
Johnson Larry
Leath Richard A.
Mezei Louis M.
Mullis Kary B.
Wennberg Timothy J.
Ferrara Richard P.
Hone William J.
Sisson Bradley L.
The Perkin-Elmer Corporation
LandOfFree
System for automated performance of the polymerase chain reactio does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System for automated performance of the polymerase chain reactio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for automated performance of the polymerase chain reactio will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-159717