Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-03-06
1999-05-04
Redding, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435383, 435395, 435420, C12Q 124
Patent
active
059003618
ABSTRACT:
Disclosed is a method and apparatus for rapidly positioning biological material in a desired orientation to form two-dimensional and three-dimensional arrays and structures. In particular, a technique is disclosed for utilizing a base material and a fixing medium that contains biological material, such that the fixing medium may fix the position of the biological material it contains upon contact with the base material. For example, a collagen suspension may be utilized as the base material, and a gelatin suspension may be used as the fixing medium, and the temperatures at which the collagen and gelatin suspensions are maintained may be controlled to allow the rapid formation of stacked cell layers. Alternative materials are also disclosed for use as the base material and the fixing medium. Also, disclosed are a method and apparatus for the precise positioning of cells into highly organized tissue structures, which are suitable for use in the treatment of burn patients. Specific placement of cells onto substrata is obtained by the computer-controlled direct placement of cell suspension matrix material, which includes cells suspended by an extracellular matrix material, including cell adhesion proteins, collagens, gelatin (i.e., degraded collagen), and cyanoacrylates or other cellular glues. A fluorescence activated cell sorter is used to provide a stream or droplet stream of the biological material. Cells may also be positioned by means of computer-controlled placement of cell adhesion materials such as fibronectin to a substratum which is suitable for maintaining cell growth, followed by allowing cells to attach to those areas of the substratum where the cell adhesion material has been placed. These apparatuses may even be further modified and improved by providing a controlled biological environment in the area of the substratum, thus providing an integral unit for the production of organized tissues.
REFERENCES:
patent: 5108926 (1992-04-01), Klebe
patent: 5641644 (1997-06-01), Klebe
Board of Regents , The University of Texas System
Redding David A.
LandOfFree
System and method for rapid positioning of cells on a substratum does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System and method for rapid positioning of cells on a substratum, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System and method for rapid positioning of cells on a substratum will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1869409