Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-14
1997-04-22
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, C12N 1510, C12Q 168
Patent
active
056228236
ABSTRACT:
The present invention pertains to a process for efficiently and accurately constructing indirectly high-resolution maps of genomes and other entities. Specifically, the process comprises the steps of generating three sets of reagents, namely, (1) sample points, (2) covering regions, and (3) probes; performing parallel experiments to efficiently compare these reagents and acquire two data tables, one of (a) probes with covering regions, and another of (b) covering regions with sample points; combining these two independent tables by means of an inner product operation to produce a third table that indirectly compares probes with sample points; analyzing this computed table to construct a high-resolution map of the probes, the sample points, and the covering regions. The resulting integrated map is then used to efficiently search for probes based on their proximity to selected sample points. With genome maps, this search can enable the rapid discovery of genes, their products, and their useful applications.
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Cox et al. (1990), Science 250: 245-250.
Mott et al. (1993) Nucleic Acids Research, vol. 21, pp. 1965-1974.
Green et al. (1991) PCR Methods and Applications, vol. 1, pp. 77-90.
Wilson (1992) Cytogenetics and Cell Genetics, vol. 59, pp. 110-111.
Monaco et al. (1991) Nucleic Acids Research, vol. 19, pp. 3315-3318.
Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring HarborLab, Cold Spring Harbor) pp. 9.1-9.62.
Altherr et al. (1992) Genomics, vol. 13, pp. 1040-1046.
Carter Philip W.
Guzo David
Schwartz Ansel M.
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