Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1996-11-13
1998-06-30
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 210659, 435 6, 536 231, 935 18, 935 77, B01D 1508
Patent
active
057728895
ABSTRACT:
Improved liquid chromatography systems having components made of titanium, coated stainless steel, or organic polymeric material are useful in the separation of nucleic acid fragments, particularly large fragments of double-stranded nucleic acids, by ion pairing reverse phase chromatography. The titanium, coated stainless steel, or polymeric components do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column may be employed to remove multivalent ions from the system. The multivalent cation capture resin may be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of nucleic acid fragments into fractions based on their molecular weight by ion pairing reverse phase chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
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Gjerde Douglas T.
Haefele Robert M.
Togami David W.
Therkorn Ernest G.
Transgenomic Inc.
Walker William B.
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