Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-03-01
2005-03-01
McKelvey, Terry (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S252300, C435S071200
Reexamination Certificate
active
06861258
ABSTRACT:
A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.
REFERENCES:
patent: 5491079 (1996-02-01), Knol et al.
patent: 6344327 (2002-02-01), Peredultchuk et al.
patent: 6350591 (2002-02-01), Weber et al.
Kayser et al., J. Bacteriol., vol. 183, No. 5, pp. 1792-1795 (2001).*
Tamakoshi et al., J. Bacterol. 179 (15), pp. 4811-4814 (1997).*
Mollet et al., J. Bacteriol. 175 (14), pp. 4315-4324 (1993).*
Nishiyama et al., J. Biol. Chem, 261 (30), 14178-14183 (1986).
Kayser Kevin J.
Kilbane II John J.
Park Ho-Shin
Fejer Mark E.
Gas Technology Institute
McKelvey Terry
Vogel Nancy T.
LandOfFree
System and method for introduction and stabilization of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System and method for introduction and stabilization of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System and method for introduction and stabilization of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3369956