System and method for introduction and stabilization of...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S252300, C435S071200

Reexamination Certificate

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06861258

ABSTRACT:
A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.

REFERENCES:
patent: 5491079 (1996-02-01), Knol et al.
patent: 6344327 (2002-02-01), Peredultchuk et al.
patent: 6350591 (2002-02-01), Weber et al.
Kayser et al., J. Bacteriol., vol. 183, No. 5, pp. 1792-1795 (2001).*
Tamakoshi et al., J. Bacterol. 179 (15), pp. 4811-4814 (1997).*
Mollet et al., J. Bacteriol. 175 (14), pp. 4315-4324 (1993).*
Nishiyama et al., J. Biol. Chem, 261 (30), 14178-14183 (1986).

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