System and method for increasing the contrast of an image...

Optical: systems and elements – Compound lens system – Microscope

Reexamination Certificate

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C356S244000

Reexamination Certificate

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06956695

ABSTRACT:
The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film behind the sample. The reflecting film reflects the emission light emitted by the fluorescent tags in the sample back through the objective lens while allowing the shorter wavelength excitation light to pass through the sample holder.

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