Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...
Reexamination Certificate
2003-06-24
2008-09-09
Lankford, L. Blaine (Department: 1651)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Solid support and method of culturing cells on said solid...
C435S325000, C435S283100, C435S305100, C435S402000, C435S177000, C435S182000, C623S013110, C623S013170, C623S013140
Reexamination Certificate
active
07422900
ABSTRACT:
A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.
REFERENCES:
patent: 4605623 (1986-08-01), Malette et al.
patent: 4642292 (1987-02-01), Reid et al.
patent: 4801299 (1989-01-01), Brendel et al.
patent: 4940853 (1990-07-01), Vandenburgh
patent: 5153136 (1992-10-01), Vandenburgh
patent: 5443950 (1995-08-01), Naughton et al.
patent: 5618718 (1997-04-01), Auger et al.
patent: 5756350 (1998-05-01), Lee et al.
patent: 6114164 (2000-09-01), Dennis et al.
patent: 6207451 (2001-03-01), Dennis et al.
patent: 6303286 (2001-10-01), Dennis et al.
patent: 6448076 (2002-09-01), Dennis et al.
Nordin, M. et al., Biomechanics of Tendons and Ligaments, In: Nordin, M. nd Frankel V.H., eds. Basic Biomechanics of the Musculoskeletal System, New York: Lippincott Williams & Wilkins, 2001, pp. 102-125.
Cao, Y.L. et al., Bridging Tendon Defects Using Autologous Tenocyte Engineered Tendon in a Hen Model, Plastic and Reconstructive Surgery, 110, 1280, 2002.
Bell, E. et al., Production of a Tissue-Like Structure by Contraction of Collagen Lattices by Human-Fibroblasts of Different Proliferative Potential In Vitro, Proceedings of the National Academy of Sciences of the United States of America, 76, 1274, 1979.
Brown, R.A. et al., Tensional Homeostatis in Dermal Fibroblasts: Mechanical Responses to Mechanical Loading in Three-Dimensional Substrates, Journal of Cellular Physiology, 175, 323, 1998.
Cacou, C. et al., A System for Monitoring the Response of Uniaxial Strain on Cell Seeded Collagen Gels, Medical Engineering & Physics, 22, 327, 2000.
Dennis, R.G. and Kosnik, P.E. Mesenchymal Cell Culture: Instrumentation and Methods for Evaluating Engineered Muscle. In: Atala, A. and Lanza R.P., eds. Methods of Tissue Engineering. New York: Academic Press, 2002, pp. 307-315.
Fung, Y.C., Biomechanics: Mechanical Properties of Living Tissues. New York: Springer, 1993, pp. 252-263.
Parry, D.A.D.and Craig, A.S., Growth and Development of Collagen Fibrils in Connective Tissue. In: Ruggeri, A. and Motta, P.M. eds. Ultrastructure of the Connective Tissue Matrix. Boston: M. Nijhoff Publishers, 1984, pp. 34-64.
Vandenburgh et al., Skeletal Muscle Growth is Stimulated by Intermittent Stretch-Relaxation in Tissue Culture, American Psych, Society, 1989, pp. C674-C682.
Vandenburgh, A Computerized Mechanical Cell Stimulator for Tissue Culture Effects on Skeletal Muscle Organogensis, In Vitro Cellular & Development Biology, vol. 24, No. 7, Jul. 1988, pp. 609-619.
Vandenburgh et al. Longitudinal Growth of Skeletal Myotubes In Vitro in a New Horizontal Mechanical Cell Stimulator, In Vitro Cell Dev. Bio., vol. 25, No. 7, Jul. 1989, pp. 607-616.
Vandenburgh et al., Computer-Aided Mechanogenesis of Skeletal Muscle Organs from Single Cells In Vitro, The FASEB Journal, vol. 5, Oct. 1991, pp. 2860-2867.
Vandenburgh et al., Tissue-Engineered Skeletal Muscle Organoids for Reversible Gene Therapy, Human Gene Therapy, Nov. 1996, pp. 2195-2200.
Butler, D.L. and Awad, H.A., Perspectives on Cell and Collagen Composites for Tendon Repair, Clinical Orthopaedics and Related Research, 367, S324-S332, 1999.
Goldstein, J.D. et al., Development of a Reconstituted Collagen Tendon Prosthesis—A Preliminary Implantation Study, Journal of Bone and Joint Surgery-American vol. 71A, 1183-1191, 1989.
Iannace, S., Mechanical-Behavior of Composite Artificial Tendons and Ligaments, Biomaterials, 16, 675-680, 1995.
Shansky et al., Letter to the Editor: A Simplified Method for Tissue Engineering Skeletal Muscule Organoids In Vitro, In Vitro Cell. Dev. Biol., Oct. 1997, pp. 659-661.
McBride, D.J. et al., Morphological Characterization of Tendon Development During Chick Embryogenesis—Measurement of Birefringence Retardation, International Journal of Biological Macromolecules, 7, 71-76, 1985.
Torres, D.S. et al., Tendon Cell Contraction of Colagen-GAG Matrices In Vitro: Effect of Cross-Linking, Biomaterials, 21, 1607-1619, 2000.
Koob, T.J. and Hernandez, D.J., Material Properties of Polymerized NDGA-Collagen Composite Fibers: Development of Biologically Based Tendon Constructs, Biomaterials, 23, 203-212, 2002.
Huang, D. et al., Mechanisms and Dynamics of Mechanical Strengthening in Ligament-Equivalent Fibroblast-Populated Collagen Matrices, Annals of Biomedical Engineering, 21, 289-305, 1993.
Wakatsuki, T. et al., Cell Mechanics Studied by a Reconstituted Model Tissue, Biophysical Journal, 79, 2353-2368, 2000.
Seliktar, D. et al., Dynamic Mechanical conditioning of Collagen-Gel Blood Vessel Constructs Induces Remodeling In vitro, Annals of Biomedical Engineering, 28, 351-362, 2000.
Holmes, D.F. et al., Reconstitution of Collagen Fibrils In Vitro—The Assembly Process Depends on the Initiating Procedure, International Journal of Biological Macromolecules, 8, 161-166, 1986.
Birk, D.E. and Zycband, E., Assembly of the Tendon Extracellular Matrix During Development, Journal of Anatomy, 184, 457-463, 1994.
Nurminskaya, M.V. and Birk, D.E. Differential Expression of Genes Associated with Collagen Fibril Growth in the Chicken Tendon: Identification of Structural and Regulatory Genes by Subtractive Hybridization, Archives of Biochemistry and Biophysics, 350, 1-9, 1998.
Murrell, G.A.C. et al., Effects of Immobilization on Achilles-Tendon Healing in a Rat Model, Journal of Orthopaedic Research, 12, 582-591, 1994.
Gelberman, R.H. et al., The Early States of Flexor Tendon Healing—A Morphologic Study of the First 14 Days, Journal of Hand Surgery-American vol., 10A, 776-784, 1985.
Dennis, R.G. and Kosnik, P.E., Excitability and Isometric Contractile Properties of Mammalian Skeletal Muscle Constructs Engineered In Vitro, In Vitro Cellular Development Biology-Animal, 36, 327-335, 2000.
Kurata, S. and Hata, R., Epidermal Growth-Factor Inhibits Transcription of Type-I Collagen Genes and Production of Type-I Collagen in Cultured Human Skin Fibroblasts in the Presence and Absence of L-Ascorbic Acid 2-Phosphate, A Long-Acting Vitamin-C Derivatie, Journal of Biological Chemistry, 266, 9997-10003, 1991.
Betsch, D.F. and Baer, E., Structure and Mechanical Properties of Rat Tail Tendon, Biorheology, 17, 83-94, 1980.
McBride, D.J. et al., Structural and Mechanical Assessment of Developing Chick Tendon, International Journal of Biological Macromolecules, 10, 194-2000, 1988.
Haut, T.L. and Haut, R.C., The State of Tissue Hydration Determines the Strain-Rate-Sensitive Stiffness of Human Patellar Tendon, Journal of Biomechanics, 30, 79-81, 1997.
Kang, H.J. and Kang, E.S., Ideal Concentration of Growth Factors in Rabbit's Flexor tendon Culture, Yonsei Medical Journal, 40, 26-29, 1999.
Ishikawa, O. et al., Morphological and Biochemical Analyses on Fibroblasts and Self-Produced Collagens in a Novel Three-Dimensional Culture, British Journal of Dermatology, 136, 6-11, 1997.
Hall, B.K. and Herring, S.W., Paralysis and Growth of the Musculoskeletal in the Embryonic Chick, Journal of Morphology, 206, 45-56, 1990.
Germiller, J.A. et al., Muscle and Tendon Size Relationships in a Paralyzed Chick Embryo Model of Clubfoot, Journal of Pediatric Orthopaedics, 18, 314-318, 1998.
Beckham, C. et al., Role of Movement in Development of a Digital Flexor Tendon, American Journal of Anatomy, 150, 443-460, 1977.
Postacchini, F. and DeMartino, C., Regeneration of Rabbit Calcaneal Tendon Maturation of Collagen and Elastic Fibers Following Partial Tenotomy, Connective Tissue Research, 8, 41-47, 1980.
Wantanabe, M. et al., Maturation-Related Biochemical-Changes i
Arruda Ellen M.
Calve Sarah C.
Dennis Robert G.
Kosnik Paul E.
Brooks & Kushman P.C.
Lankford L. Blaine
The Regents of the University of Michigan
LandOfFree
System and method for forming a connective tissue construct does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System and method for forming a connective tissue construct, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System and method for forming a connective tissue construct will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3992079