System and method for electroporating a sample

Details System and method for electroporating a sample System and method for electroporating a sample

2005-05-24

2005-05-24

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S285200

Reexamination Certificate

active

06897069

ABSTRACT:
A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.

REFERENCES:
patent: 6241701 (2001-06-01), Hofmann
patent: 6352853 (2002-03-01), King et al.
patent: 6628382 (2003-09-01), Robertson
patent: 03057819 (2003-07-01), None
Joseph R. Speyer, “Multi-Sample Electroporation”, BioTechniques, BioFeedback-Benchmarks, vol. 8, No. 5, May 1990.
Joseph R. Speyer, “A Simple and Effective Electroporation Apparatus”, BioTechniques—BioFeedback, vol. 8, No. 1, 1990.

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