System and method for detecting and classifying biological...

Optics: measuring and testing – Plural test

Reexamination Certificate

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C356S343000, C356S344000, C250S492100, C436S172000

Reexamination Certificate

active

06885440

ABSTRACT:
A continuous wave laser excites a biological particle. Detection channels are created to detect light scattered by the biological particle, and to detect any auto-fluorescence emitted by the biological particle. Additional channels can also detect light emitted by auto-fluorescence of the biological particle when simultaneously excited by light at harmonics of the laser's fundamental wavelength. The biological particle is identified using Mie scattering and auto-fluorescence. Ratio-metric calculations generated by calculating ratios of detected peak heights or integrated pulse values in the channels provides additional information for identifying and classifying the biological particle. A warning or alert can be provided if the identified biological particle is a particle of interest.

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