System and method for calibrating a fluorescence microscope

Details System and method for calibrating a fluorescence microscope System and method for calibrating a fluorescence microscope

2007-04-03

2007-04-03

Ullah, Akm (Department: 2809)

Radiant energy

Calibration or standardization methods

C250S458100, C378S044000

Reexamination Certificate

active

11331961

ABSTRACT:
A system and method are provided to calibrate fluorescence detection of a fluorescence microscope by using a near-perfectly uniform reflector as a target in combination with temporary removal of the microscope's emission filter. Excitation light is reflected from the near-perfectly uniform reflector back into the microscope's objective optical system and transmitted to a dichroic. A small fraction of the excitation light passes though the dichroic and is measured by a CCD camera or other appropriate measurement device. By measuring the intensity of the residual excitation light at a plurality of points in the field of view, variations in illumination intensity may be determined. Using this, fluorescence detection at different points in the field of view may be readily calibrated.

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