System and method employing photokinetic techniques in cell...

Details System and method employing photokinetic techniques in cell... System and method employing photokinetic techniques in cell...

2008-08-04

2009-10-27

Porta, David P (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

C250S458100

Reexamination Certificate

active

07608840

ABSTRACT:
A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

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