System and method employing photokinetic techniques in cell...

Details System and method employing photokinetic techniques in cell... System and method employing photokinetic techniques in cell...

2004-06-18

2008-08-05

Porta, David P. (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

C250S458100

Reexamination Certificate

active

07408176

ABSTRACT:
A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

REFERENCES:
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Sibarita, J-B, et al., Ultra-fast 4D microscopy and high throughput distributed deconvolution, 2002 IEEE International Symposium on Biomedical Imaging, Jul. 7, 2002, pp. 769-772.
Kato, Naohiro, Structureal analyses of living plant nuclei, Genetic Engineering, 23:65-90 (2003).
Tsien R.Y: The green fluorescent protein, Annual Review of Biochemistry, 67:509-544 (1998).

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