Synthetic yeast leader peptides

Details Synthetic yeast leader peptides Synthetic yeast leader peptides

1992-11-09

1994-05-31

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

530324, 4353201, 536 237, C07K 710

Patent

active

053169239

ABSTRACT:
Synthetic leaders for effective secretion of proteins in yeast are provided. Also provided are replicable yeast vectors containing a DNA-sequence encoding the synthetic leader positioned upstream to a DNA-sequence encoding the desired product and operably connected with promoter and signal sequences. There are also provided yeast strains transformed with such vectors and a method for producing proteins by means of the transformed yeast strains.

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Egel-Mitani et al., Nucleic Acids Research, vol. 15, No. 15, pp. 6303-6304 (1987).
Egel-Mitani et al., Gene, vol. 73, pp. 113-120 (1988).
Kaiser et al., Research Articles, vol. 235, pp. 312-317 (1987).
Andrews et al., J. Biol. Chem., vol. 263, No. 30, pp. 15791-15798 (1988).
Duffaud et al., J. Biol. Chem., vol. 263, No. 21, pp. 10224-10228 (1988).

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