Synthetic urogastrone gene, corresponding plasmid recombinants,

Chemistry: molecular biology and microbiology – Vector – per se

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435 70, 435 71, 435 91, 4351723, 435 253, 536 27, 935 13, 935 16, 935 17, 935 29, 935 73, C12N 100, C12N 1500, C12N 120, C12P 2102, C12P 2104, C12P 1934, C07H 2104

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active

047191805

ABSTRACT:
A synthetic gene characterised in that it codes for the expression of urogastrone or a sub-unit thereof is disclosed.
The production thereof by the assembly and ligation of a number of nucleotide blocks is also disclosed, as are corresponding plasmid recombinants, transformed cells and the production thereof.
The expression of urogastrone is further disclosed.
Urogastrone is a polypeptide hormore (protein) which may be isolated in small amounts from human urine. It has an application in the treatment of ulcers and in the promotion of wound healing. The present invention provides a more viable commercial production.

REFERENCES:
patent: 4293652 (1981-10-01), Cohen
patent: 4322499 (1982-03-01), Baxter et al.
patent: 4342832 (1982-08-01), Goeddel et al.
Itakura et al., "Expression in Escherichi coli of a Chemically Synthesized Gene for the Hormone Somatostatin", Science 196: 1056 (1977).
Lewin, "Gene Expression, vol. 2, (1974), John Wiley & Sons, London, pp. 148-156.

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