Synthetic polypeptide having fish growth hormone-like...

Details Synthetic polypeptide having fish growth hormone-like... Synthetic polypeptide having fish growth hormone-like...

1998-11-24

2001-05-29

Mertz, Prema (Department: 1646)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C530S350000, C530S399000, C536S023100, C536S023500, C536S023510, C435S069400, C435S071100, C435S071200, C435S471000, C435S325000, C435S375000, C435S252300, C435S254110, C514S008100, C514S012200, C424S093100, C424S093200, C424S439000, C424S442000

Reexamination Certificate

active

06239100

ABSTRACT:

CROSS REFERENCE TO RELATED APPLICATION
This is a §371 of International PCT Application, PCT/JP98/01230, filed Mar. 23, 1998, and published under number WO98/42748.
TECHNICAL FIELD
This invention relates to a novel polypeptide, and more specifically to a polypeptide which shows fish growth hormone-like activity, a DNA which codes thereof, a vector which contains said DNA, a microorganism which is transformed with said vector, a preparation process of the above-mentioned peptide using said microorganism, and use of said peptide in promoting fish growth and reducing mortality of fry.
BACKGROUND ART
Growth hormones the existence of which is common to mammals, avians, reptiles, amphibians and fish are polypeptides that are secreted from the pituitary gland, and there are various reports about growth hormones that were isolated and the structure of which was determined by various research groups. Along with advances in gene engineering, mass production of these growth hormones in microorganisms has become possible and is about to be used for many kinds of livestock. Concerning fish the current situation, however, is that growth hormones are only used on research basis but not applied for practical use.
Since fish growth hormones are responsible for growth promotion of fish, it is believed that if fish growth hormones were available as fodder for hatchery fish, pre-shipment period could be shortened and that this also would be helpful for cost reduction. Natural growth hormones which have been isolated from fish, however, have species specificity to some extent, and therefore, for example, when tuna growth hormone which is commercially available as an agent is given orally to fish other than tuna, although a slight effect may be expected, the effect can not be regarded as sufficient.
Therefore, the inventors of the present application did research for developing a polypeptide which can be used for a variety of fish having universal fish growth hormone-like activity, by exerting supreme growth-promoting activity in fish.
DISCLOSURE OF INVENTION
As a result, it was found that a polypeptide with the following amino acid sequence (SEQ ID NO: 1)
Gln Pro Ile Thr Asp Ser Gln Arg Leu Phe Ser Ile Ala Val Ser Arg
  1               5                  10                  15
Val Gln His Leu His Leu Leu Ala Gln Arg Leu Phe Ser Asp Phe Glu
             20                  25                  30
Ser Ser Leu Gln Thr Glu Glu Gln Arg Gln Leu Asn Lys Ile Phe Leu
         35                  40                  45
Gln Asp Phe Cys Asn Ser Asp Tyr Ile Ile Ser Pro Ile Asp Lys His
     50                  55                  60
Glu Thr Gln Arg Ser Ser Val Leu Lys Leu Leu Ser Ile Ser Tyr Arg
 65                  70                  75                  80
Leu Val Glu Ser Trp Glu Phe Pro Ser Arg Ser Leu Ser Gly Gly Ser
                 85                  90                  95
Ala Pro Arg Asn Gln Ile Ser Pro Lys Leu Ser Glu Leu Lys Met Gly
            100                 105                 110
Ile His Leu Leu Ile Arg Ala Asn Gln Asp Gly Ala Glu Met Phe Ala
        115                 120                 125
Asp Ser Ser Ala Leu Gln Leu Ala Pro Tyr Gly Asn Tyr Tyr Gln Ser
    130                 135                 140
Leu Gly Gly Asp Glu Ser Leu Arg Arg Asn Tyr Glu Leu Leu Ala Cys
145                 150                 155                 160
Phe Lys Lys Asp Met His Lys Val Glu Thr Tyr Leu Arg Val Ala Lys
                165                 170                 175
Cys Arg Leu Ser Pro Glu Ala Asn Cys Thr Leu
            180                 185
shows growth hormone-like activity, and displays remarkable growth-promoting and fry mortality-reducing effects in a variety of fish.
Therefore, the aim of this invention is to provide a polypeptide which comprises the above-mentioned amino acid sequence, or an amino acid sequence of which one or more amino acid(s) of the above-mentioned amino acid sequence is/are deleted, substituted or added, and which also shows fish growth hormone activity.
The polypeptide of this invention can be chemically synthesized by a solid phase method or a liquid phase method which is known per se, or biochemically synthesized by means of gene engineering technique using a DNA which codes the polypeptide of this invention.


REFERENCES:
patent: 2-223596 (1990-09-01), None
patent: 5-568572 (1993-03-01), None
Noso, et al., Isolation and characterization of growth hormone . . . , Int. J. Peptide Protein Res., vol. 32, 1988, pp. 579-589.
Kariya, et al., Isolation and characterization of growth hormone . . . , Agric. Biol. Chem., vol. 53, No. 6, 1989, pp. 1679-1687.
Sato et al. (1988) Biochimica et Biophysica Acta 949 (1988) pp. 35-42.*
Noso et al., (1988) Int. J Peptide Protein Res. vol. 32, pp. 579-589.*
George et al., (1988)

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