Synthetic plasmid and bacteria containing it

Details Synthetic plasmid and bacteria containing it Synthetic plasmid and bacteria containing it

1981-11-05

1986-01-28

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435128, 435253, 435878, 435317, 435 29, 435 56, 435 72, C12N 1500, C12N 120, C12N 100, C12P 1300, C12R 141

Patent

active

045671460

ABSTRACT:
Bacteria of the genus Rhizobium nodulate legumes and are responsible for nitrogen fixation. The energetics of this process are improved if hydrogen uptake ability (Hup) is imparted to the bacteria to "recycle" some of the hydrogen lost during the nitrogen fixation. Such Hup is observed in a naturally occurring strain of Rhizobium leguminosarum but it was not known how to transfer it to other strains. The present invention provides recombinant plasmids pIJ1008 and pIJ1007 which enable it to be transferred. These plasmids are formed from a transmissible plasmid, synthesized to include a drug-resistance marker, and a plasmid of the naturally occurring strain. Cultures containing the plasmids have been deposited in a culture collection and can be used, after appropriate further crosses as described, to impart Hup to other strains of Rhizobium. In addition, cultures containing the starting transmissible plasmids (pVW5JI and pVW3JI) have been deposited and can be used to prepare strains of Rhizobium containing the same or similar recombinant plasmids. Plant growth analysis in laboratory tests demonstrate that rhizobial strains containing these recombinant nodulation plasmids which confer Hup.sup.+ ability are superior to the corresponding Rhizobium field isolates in terms of overall plant growth dependent on symbiotic nitrogen fixation.

REFERENCES:
Brewin et al.: Nature 288, 77 (1980).
Brewin et al.: J. Gen. Microbiol. 116, 261 (1980).
Lim et al.: Trends in Biochemical Sciences, Jun. 1980, pp. 167-170.
Johnston et al.; in Plasmids of Medical, Environmental and Commercial Importance, Timmis et al., (ed.), Elsevier/North Holland, 1979, pp. 317-325.
Buchanan-Wollaston et al.; Molec. Gen. Genet. 178, 185 (1980).
Evans et al.; In Report of the Public Meeting on Genetic Engineering for Nitrogen Fixation, National Academy of Sciences, U.S. Govt. Printing Office, 1977, pp. 61-76.

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