Synthetic peptides specific for the detection of antibodies to H

Details Synthetic peptides specific for the detection of antibodies to H Synthetic peptides specific for the detection of antibodies to H

1991-12-11

1995-07-25

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 792, 435968, 436518, 436533, 436820, C12Q 170

Patent

active

054361265

ABSTRACT:
The present invention relates to a method for the detection in body fluids of antibodies to hepatitis C virus (HCV), also known as a non-A non-B hepatitis (NANBH) virus and to the diagnosis of NANBH by the use of a composition of synthetic peptides. Each of these peptides has an amino acid sequence corresponding to immunodominant regions of a fusion protein and a non-structural polypeptide of HCV, SOD/HCV C100 and a postulated HCV structural (core) protein. More specifically, the present invention is directed to the use of a group of synthetic peptides in a prescribed sequence or their analogues for the detection of antibodies to HCV in body fluids. The detection method includes an enzyme-linked immunosorbent assay (ELISA), and other forms of immunoassay procedures. The present invention also relates to a method for generating high titer antibodies to HCV in healthy mammals, including humans, by the use of compositions containing these synthetic peptides, analogues or mixtures thereof, in a free, conjugated or polymeric form as key components in synthetic vaccines for the prevention of non-A non-B hepatitis (NANBH).

REFERENCES:
patent: 4356164 (1982-10-01), Tabor et al.
patent: 4395395 (1983-07-01), Tabor et al.
patent: 4464474 (1984-08-01), Coursaget et al.
patent: 4542016 (1985-09-01), Trepo
patent: 4554101 (1985-11-01), Hopp
patent: 4673634 (1987-06-01), Seto et al.
patent: 4702909 (1987-10-01), Villarejos et al.
patent: 4735896 (1988-04-01), Wang et al.
patent: 4777245 (1988-10-01), Foung et al.
patent: 4833071 (1989-05-01), Wang et al.
patent: 4871659 (1989-10-01), Pillot
patent: 4879212 (1989-07-01), Wang et al.
MacCullum F.O., et al.; Lancet, 1:622 (1944).
Havens, W. P.: Proc Soc Exp Biol Med, 59:148 (1945).
Krugman, S. et al.: JAMA, 200:365 (1967).
Prince A. M.: Lancet, 2:241 (1974).
Alter H. J., et al: Lancet, 2:838 (1975).
Galbraith, R. M., et al: Lancet, 2:886 (1975).
Mosley, J. W., et al: N. Engl J Med, 296:75 (1977).
Dienstag, J. L.: Rush-Presbyterian-St. Luke's Med Bull, 15:104 (1976).
Asch, R. D., et al: N. Engl J Med 304:989 (1981).
Hollinger F. B., et al: Viral Hepatitis: 1981 International Symposium, Szmuness, W., Alter, H. J., Maynard, J. E., (eds), Philadelphia: Franklin Institute Press, p. 361 (1982).
Alter, H. J., et al.: JAMA, 246:630 (1981).
Stevens, C. E., et al: Ann Int. Med, 101:733 (1984).
Koziol, D. E., et al: Ann Int Med, 104:488 (1986).
Sugg, U., et al.: Transfusion, 28:386 (1988).
Choo Q-L., et al: Science, 244:359 (1989).
Kuo, G., et al: Science, 244:362 (1989).
Wang, C. Y.: Synthetic Peptides in Biotechnology, A. Mizrahi (3d), Adv in Biotechnological Processes, 10:131 (1988).
Wang, J. G., et al.: Proc Natl Acad Sci USA, 83:6159 (1986).
UBI-Olympus HIV-1 EIA Product Insert, Jun. 1, 1989. License No. 1079, approved by U.S. FDA.
Okamoto, H., et al.: Jpn. J. Exp. Med. 60(3):167-177 1990.
Schlesinger, S., et al.: The Togaviridae and Flaviviridae. In: The Viruses, Plenum Press, N.Y., 1986.
Kubo, Y., et al: Nucleic Acid Res. 17:10367-10372 (1989).
Slide Presentation by Abbot Laboratories to American Association of Blood Banks (Oct. 1989).
Weiner et al., Lancet, 335: 1-3 (Jan. 1990).
McClelland et al., Lancet, pp. 36-37 (Jul. 1987).
Feinman et al., CMA Journal, 123: 181-184 (Aug. 1980).
T. H. Maugh II, Science, 210: 999-1000 (Nov. 1980).
Hantz et al., J. Med. Virol., 5:73-86 (1980).
Tabor et al., N. Engl. J. of Med., 303: 139-143 (1980).
Letters to Editor, Lancet, pp. 796-799 (Sep. 30, 1989).
Lynn et al., MMWR, 38:529-531 (Aug. 1989).
Polesky et al., Arch. Pathol. lab. Med., 113:232-235 (Mar. 1989).
G. Kolata, Science, 23:149-150 (Jul. 1986).
Troisi et al., Transfusion, 27:438-440 (1987).
Schumacher et al., The Ligand Quart., 5: 12-27 (Nov. 1982).
Arima T. et al., Gastroenterologia Jap., 24:540-544 (1989).
Arima T. et al., Gastroenterologia Jap., 24:545-548 (1989).
Arima T. et al., Gastroeneterologia Jap., 24:685-691 (1989).
Arima T. et al., Gastroenterologia Jap., 25:2218-232 (1990).
Chou, P. Y. and Fasman, G. D., Biochemistry, 13:222-245 (1974).

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