Synthetic peptides for the production of specific keratin protei

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435 70, 4351723, 435 7, 935 12, C12P 2100, C12P 2102, C12N 1500, C12Q 100

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047228954

ABSTRACT:
A process is disclosed for the construction of synthetic peptides corresponding to amino acid sequences of 55-, 59-, 60-, 67-kilodalton keratin proteins. These synthetic peptides make possible the development of monospecific antisera for individual keratin proteins. The process involves preparing cDNA libraries and reducing the amino sequences of cDNA clones.

REFERENCES:
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Roop et al., Proc. Nat'l. Acad. Sci. (USA) 80(3): 716-720, 1983.
Roop et al., J. Biol. Chem. 259 (13): 8037-8040, 1984.
Goodman et al., in Methods in Enzymology, vol. 68: 75-90, 1979.
Roop et al., PNAS, vol. 80, p. 716 (Feb. 1983).
Steinert et al., Nature, vol. 302, p. 794 (Apr. 1983).
Steinert et al., Biochim. Biophys. Acta, vol. 577, p. 11 (1979).

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