Synthetic peptides, antibodies against them and their use

Details Synthetic peptides, antibodies against them and their use Synthetic peptides, antibodies against them and their use

1996-10-08

1999-11-09

Minnifield, Nita

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

530324, 530325, 530326, 530327, 530330, 530333, 530381, 530382, 930100, A61K 3800, C07K 200, C07K 500, C07K 1600

Patent

active

059816970

ABSTRACT:
The present invention is directed to the generation of antibodies which preferentially bind to fibrinogen fragments E1, E2, and E3, but exhibit little or no cross-reactivity against fibrin monomer and fibrinogen. Thus, the invention provides synthetic peptides containing defined amino acid sequences corresponding to the carboxy terminal regions of the E fragments which arise as a result of plasmin cleavage of fibrin and fibrinogen. The synthetic peptides may be synthesized chemically, or through genetic manipulations, and may contain additional amino acid sequences which are not contiguous with the defined E fragment sequences.

REFERENCES:
patent: 4427646 (1984-01-01), Olexa et al.
patent: 4960712 (1990-10-01), Theofilopoulos et al.
patent: 5143838 (1992-09-01), Kraus et al.
patent: 5151412 (1992-09-01), Brown
patent: 5288490 (1994-02-01), Budzynski et al.
patent: 5478810 (1995-12-01), Stuber et al.
patent: 5599678 (1997-02-01), Kraus et al.
patent: 5607858 (1997-03-01), Stuber et al.
Hui et al, Science, vol. 222:1129-1132, Dec., 1983.
Canfield et al, Biochem., 15/6:1203-1209, 1976.
Takagi et al, Biochem., 14/5:940-946, 1975.
Ming Ge, et al, Am. J. Physiol. 261(4pt1): L283-L289, 1991.
Canfield et al, Biochem., 15/6:1203-1209 1976.
Hoeprich et al, Biochem., 22:2049-2055, 1983.
Kirschbaum et al., "A Unique Proteolytic Fragment of Human Fibrinogen Containing the A.alpha. COOH-terminal Domain of the Native Molecule," J. Biol. Chem. 265(23):13669-75 (1990).
Koppert et al., "A Monoclonal Antibody-Based Enzyme Immunoassay for Fibrin Degradation Products in Plasma," J. Int.'l. Soc. on Thrombosis and Haemostatis 59(2):310-315 (1988).
Kapmeyer et al., "Automated Nephelometric Immunoassays With Novel Shell/Core Particles," J. Clin. Lab. Analysis 2:76-83 (1988).
Nieuwenhiuzen, "Plasma Assays of Fibrinogen/Fibrin Degradation Products and Their Clinical Relevance," Fibrinogen 2:173-181 (1987).
Foster et al., "Turbidimetry," Manual of Clin. Lab. Immunology 25-109 (1986).
Gaffney et al., "Unreliability of Current Serum Fibrin Degradation Product (FDP) Assays," Thrombosis and Haemostasis 53(3):301-302 (1985).
Schifreen et al., "A Quantitative Automated Immunoassay for Fibrinogen/Fibrin Degradation Products," Clin. Chem. 31(9):1468-1473 (1985).
Gaffney et al., "Monoclonal Antibodies Against Fibrinogen, Fibrin and Their Fragments," Thrombosis and Haemostasis 54(3):733-734 (1985).
Tijssen, "Practice and Theory of Enzyme Immunoassays," Laboratory Techniques in Biochem. and Molec. Biol. 15:108-109 (1985).
Chen et al., "Radioimmunoassay of Fragment E-related Neoantigen: Validation Studies and Clinical Application," Brit. J. Haematol. 57:133-144 (1984).
Henschen et al., Covalent Structure of Fibrinogen, Annals New York Acad. of Sci. 28-43 (1983).
Hui et al., "Monoclonal Antibodies to a Synthetic Fibrin-Like Peptide Bind to Human Fibrin but Not Fibrinogen," Science 222:1129-1132 (1983).
Price et al., "Shadow-cast Electron Microscopy of Fibrinogen with Antibody Fragments Bound to Specific Regions," Proc. Natl. Acad. Sci. USA 78(1):200-204 (1981).
Sevier et al., "Monoclonal Antibodies in Clinical Immunology," Clin. Chem. 27(11):1797-1806.
Plow et al., "Localization and Characterization of the Cleavage-associated Neoantigen Locus in the E Domain of Fibrinogen," J. Biol. Chem. 254(3):672-78 (1979).
Olexa et al., "Binding of Phenomena of Isolated Unique Plasmic Degradation Products of Human Cross-linked Fibrin," J. Biol. Chem. 254(11):4925-4932 (1979).
Nakane et al., "Peroxidase-Labeled Antibody A New Method of Conjugation," J. of Histochem. and Cytochem. 22(12):1084-1091 (1974).
Mersky et al., "A Rapid, Simple, Sensitive Method for Measuring Fibrinolytic Split Products in Human Serum," 131:871-875 (1968).
Axen et al., "Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides," Nature 214:1302-1304 (1967).
Freifelder, "Physical Biochemistry," 236 (1976).
Chung et al., "Nucleotide Sequences of the Three Genes Coding for Human Fibrinogen," Fibrinogen, Thrombosis, Coagulation, and Fibronolysis Advances Exp. Med. Biol. 1990, 281:39-48.
Olexa et al., "Structure of Fragment E Species from Human Cross-Linked Fibrin," Biochem. 21:6139-45 (1981).

Affiliated with

Also associated with

Rating

Synthetic peptides, antibodies against them and their use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthetic peptides, antibodies against them and their use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic peptides, antibodies against them and their use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1457599