Synthetic methods and intermediates for triester...

Details Synthetic methods and intermediates for triester... Synthetic methods and intermediates for triester...

2005-07-19

2005-07-19

Wilson, James O. (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025310, C536S025340, C435S006120

Reexamination Certificate

active

06919437

ABSTRACT:
Methods for the preparation of oligonucleotides having bioreversible phosphate blocking groups are disclosed. In one aspect, the present invention provides compounds comprising a sequence of nucleotide units that includes a first segment having at least one internucleoside linkage of formula:in-line-formulae description="In-line Formulae" end="lead"?3′-O—P(X)(O−)—O-5′in-line-formulae description="In-line Formulae" end="tail"?and a second segment having at least one internucleoside linkage of formula:in-line-formulae description="In-line Formulae" end="lead"?3′-O—P(X)(Y1—(CH2)q—Y2—Y3—Z)—O-5′in-line-formulae description="In-line Formulae" end="tail"?wherein each of X, Y1, and Y2is, independently, O or S; q is 2 to about 4; Y3is C(═O) or S; and Z is aryl having 6 to about 14 carbon atoms or alkyl having from one to about six carbon atoms.

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