Synthetic malarial antigens and uses thereof

Details Synthetic malarial antigens and uses thereof Synthetic malarial antigens and uses thereof

1993-01-15

1995-10-10

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Amino acid sequence disclosed in whole or in part; or...

4241851, 4242651, 4242681, 4242721, 530324, 530325, 530326, 530327, 530328, 530329, A61K 39015, A61K 3808, A61K 3816

Patent

active

054569117

ABSTRACT:
The present invention includes novel peptides GQDEGEENEG, SEQ. ID NO:1 GRNGLGANTDQDDQLEDE, SEQ. ID NO:2 DQFFDANPNLFQLLEPVEFDED, SEQ. ID NO:3 and LVFLVQQPFLFVLWDQNEKFPVFMGVYDP SEQ. ID NO:3. Such peptides are useful for detecting antibodies against circulating malarial antigens. In one aspect, these peptides may be copolymerized or crosslinked to form immunogens presenting epitopes of the malarial Plasmodium falciparum antigen Pf70. Preferably at least two of the above peptides are so crosslinked. Most preferably, all four peptides are copolymerized. The crosslinking agent preferred is glutaraldehyde although, of course, other crosslinking agents such as dialdehydes of various lengths, for example, or even possibly the carbodiimides may be used. Compositions of matter comprising such peptides in crosslinked or copolymerized forms are an important part of the present invention, particularly when used as immunogens. Rabbits have been immunized with a composition of the four crosslinked peptides at a dose of 2.5 mg (administered three times). An analogous dose for larger animals should, of course, produce the same response to induce immunity to malaria. The present invention also, of course, includes a method of producing an antigen usable to generate antibodies reacting with malarial organisms, Plasmodium falciparum and Plasmodium vivax. The above copolymerizing methodology utilizing crosslinking agents with the peptides of choice is the method of production.

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