Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-06
1997-06-17
Ketter, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351721, 536 234, 536 235, 536 241, C02D 2100, C12N 1500, C07H 2104
Patent
active
056396422
ABSTRACT:
The present invention relates to a DNA expression cassette comprising the following sequence:
5'-P-SP-LS-PS-*gene*-(T).sub.i -3'
wherein
P is a promoter sequence,
SP is a DNA sequence encoding a signal peptide,
LS is a DNA sequence encoding a leader peptide of formula I:
GinProIle(Asp/Glu)(Asp/Glu)X.sup.1 (Glu/Asp)X.sup.2 AsnZ(Thr/Ser)X.sup.3 (SEQ ID NO: 77) (I)
wherein
X.sup.1 is a peptide bond or a codable amino acid;
X.sup.2 is a peptide bond, a codable amino acid or a sequence of up to 4 codable amino acids which may be the same or different;
Z is a codable amino acid except Pro; and
X.sup.3 is a sequence of from 4 to 30 codable amino acids which may be the same or different;
PS is a DNA sequence encoding a processing site;
*gene* is a DNA sequence encoding a polypeptide;
T is a terminator sequence; and
i is 0 or 1.
REFERENCES:
patent: 5037743 (1991-08-01), Welch et al.
Kjeldsen Thomas B.o slashed.rglum
Vad Knud
Brusca John S.
Ketter James
Lambiris, Esq. Elias J.
Novo Nordisk A S
Zelson Esq. Steve T.
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