Synthetic glucagon binding proteins

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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530350, 536 235, 4353201, 435325, 4352523, 43525411, 435 691, C12N 1510, C12N 510, C07K 14705

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active

058891676

ABSTRACT:
A synthetic human glucagon (hGlu) binding protein designated hGlu.DELTA.252-259 binding protein is cloned, expressed and used in an in vitro assay to screen for compounds that bind to the synthetic binding protein, including compounds that specifically stimulate or inhibit the binding of glucagon to the synthetic receptor. The invention includes the assay, the synthetic binding protein used in the assay, DNA encoding the synthetic binding protein, cells expressing the synthetic binding protein, and compounds identified through the use of the synthetic binding protein.

REFERENCES:
Chicchi, et al., "Alterations in receptor activation and divalent cation activation of agonist binding . . . ", J. Biol. Chem., 21 Mar. 1997, vol. 272, No. 12 pp. 7765-7769.
Carruthers,et al., "Synthesis and expression of a gene for the rat glucgon receptor . . . ", J. Biol. Chem., 18 Nov. 1994, vol. 269, No. 46, pp. 29321-29328.
MacNiel, et al., "Cloning and expression of a human glucagon receptor.", Biochem. Biophys. Res. Comm., 14 Jan. 1994, vol. 198, No. 1, pp. 328-334.
Unson,et al., "Characterization of deletion and truncation mutants of the rat glucagon receptor.", J. Biol. Chem., 17 Nov. 1995, vol. 270, No. 46, pp. 27720-27727. glucagon receptor expressed . . .", j. Biol. Chem., 3 Nov. 1995, vol. 270, No. 44, pp. 26466-26472.
Hamilton, et al., nursing 86 Drug Handbook, Pennsylvania, USA Springhouse Corporation 1986, pp. 494, col. 2 and p. 495, col. 1.

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