Synthetic genes for malarial proteins and methods of use

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S069300, C435S069700, C536S023500, C536S023700, C424S268100, C424S272100, C530S350000

Reexamination Certificate

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07078507

ABSTRACT:
Synthetic gene sequences encoding erythrocyte binding protein of a malaria pathogen for the expression of the erythrocyte binding protein. The codon composition of the synthetic gene sequences approximates the mammalian codon composition. The synthetic gene sequences are useful for incorporation into the DNA vaccine vectors, for the incorporation into various expression vectors for production of malaria proteins, or both. The synthetic genes may be modified to avoid post-translational modification of the encoded protein in hosts. Administration of the synthetic gene sequences, or the encoded protein, as an immunization agent is useful for induction of immunity against malaria, treatment of malaria, or both.

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Rudinger et al, in “Peptide Hormones”, edited by Parsons, J.A., University Park Press, Jun. 1976.
Biochemical and Biophysical Research Communications 1999, 261; 445-451.
GeneEmbl, Accession No. PFU27384 or PFU 78724.
Kim et al 1997, Molecular and Biochemical Parasitology 1997, 84: 241-24.
Narum et al., “Codon Optimization of Gene Fragments EncodingPlasmodium falciparumMerzoite Proteins Enhances DNA VAccine Protein Expression and Immunogenicity in Mice,”Infection and Immunity(2001) , vol. 69, No. 12, pp. 7250-7253.
Liang and Sim, 1997, “Conservation of structure and function of the erythrocyte-binding domain ofPlasmodium falciparumEBA-175,”Mol Biochem Parasitol84: 241.

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