Synthetic gene for acyl carrier protein

Details Synthetic gene for acyl carrier protein Synthetic gene for acyl carrier protein

1987-06-04

1989-12-19

Martinell, James

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4351721, 4351723, 435235, 43525233, 435320, 530 27, 935 14, C12N 910, C12N 1500, C12N 700, C12N 120

Patent

active

048882828

ABSTRACT:
A synthetic gene which encodes for acyl carrier protein (ACP) has been designed. Construction, cloning, and expression in E. coli of spinach ACP-I has been demonstrated. In vitro production of ACP by appropriate expression vectors carrying the synthetic gene will augment the meager supply of this protein. Analogous genes designed for expression of ACP in plants would be a useful tool for controlling fatty acid synthesis and metabolism.

REFERENCES:
Daniel J. Guerra et al., "Purification and Characterization of Recombinant Spinach Acyl Protein I Expressed in Escherichia coli", J. Biol. Chem., 263(9): 4386-4391 (Mar. 25, 1988).
Phillip D. Beremand et al., "Synthesis, Cloning, and Expression in Escherichia coli of a Spinach Acyl Carrier Protein-I Gene", Arch. Biochem. Biophys., 256(1): 90-600 (Jul. 1987).
T. M. Kuo et al., "The Primary Structure of Spinach Acyl Carrier Protein", Arch. Biochem. Biophys., 234(1): 290-296 (Oct. 1984).
J. B. Ohlrogge et al., "Spinach Acyl-Carrier Proteins: Structure, Regulation, and Progress Towards Cloning", Biochem. Soc. Trans., 14(3): 579-581 (Jun. 1986).
Lathe; J. Mol. Biol., 183:1 (1985).
Amann et al; Gene 40: 183 (1985).

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