Synthetic eukaryotic promoters containing two inducible elements

Details Synthetic eukaryotic promoters containing two inducible elements Synthetic eukaryotic promoters containing two inducible elements

1996-04-16

1999-03-02

Elliott, George C.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435 6, 4351723, 4353201, 536 231, 536 241, C12N 1585, C12N 1563, C07H 7104, C12Q 168

Patent

active

058770186

ABSTRACT:
Synthetic inducible eukaryotic promoters for the regulation of transcription of a gene achieve improved levels of protein expression and lower basal levels of gene expression. Such promoters contain at least two different classes of inducible elements, usually by modification of a native promoter containing one of the inducible elements by inserting the other of the inducible elements. In embodiments, additional metal responsive elements IR:Es) and/or glucocorticoid responsive elements (GREs) are provided to native promoters, particularly the hMT-IIA and MMTV-LTR promoters. One or more constitutive elements may be functionally disabled to provide the lower basal levels of gene expression.

REFERENCES:
Filmus, J. et al. "Synergistic induction of promoters containing metal-and glucocorticoid-responsive elements" Nucleic Acids Research. vol. 20, No. 11, Jun. 11, 1992, pp. 2755-2760.
Thompson, et al. "Trans-activation by thyroid hormone receptors: functional paralleles with steroid hormone receptors" -Proceedings of the National Academy of Sciences of USA, vol. 86, No. 10, May, 1989, pp. 3494-3498.
Hu et al; Molecular and Cellular Biology, vol. 10, No. 12; pp. 6141-6151, Dec., 1990.
Wright et al; Proceedings of the National Academy of Sciences USA, vol. 88, pp. 8283-8287; Oct., 1991.
Allan et al; The Journal of Biologi cal Chemistry, vol. 266, No. 9; pp. 5905-5910; Mar. 25, 1991.

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