Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-09-19
1997-11-18
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 800DIG56, 536 2371, 935 10, 935 30, A01H 500, C12N 1511, C12N 1582
Patent
active
056890524
ABSTRACT:
A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided. Also disclosed are novel structural genes which encode insecticidal proteins of B. t. k. and monocotyledonous (e.g. maize) plants containing such novel structural genes.
REFERENCES:
patent: 5082767 (1992-01-01), Hafsield et al.
Murray et al. (1989) Codon Usage in plant genes. Nucleic Acids Research vol. 17, No. 2, pp. 477-498.
Spencer et al. (1992) Segregation of transgenes in maize. Plant Molecular Biology vol. 18, pp. 201-210.
Potrykus, I. (1990) Gene Transfer ti Cereals: An Assessment. Bio/Technology, pp. 535-542.
Brown Sherri Marie
Dean Duff Allen
Fromm Michael Ernest
Sanders Patricia Rigden
Benzion Gary
Monsanto Company
LandOfFree
Synthetic DNA sequences having enhanced expression in monocotyle does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Synthetic DNA sequences having enhanced expression in monocotyle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic DNA sequences having enhanced expression in monocotyle will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1567551