Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2006-05-16
2006-05-16
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Reexamination Certificate
active
07045599
ABSTRACT:
Synthetic versions of a full length and termini truncated humanized green fluorescent protein based onPtilosarcus gurneyiare disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild typePtilosarcus gurneyiwhich has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons ofPtilosarcus gurneyifluorescent protein. The humanizedPtilosarcus gurneyigreen fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.
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Cao Longguang
Chen Yih-Tai
Northrop, Esq. Lara A.
Pietragallo Bosick & Gordon LLP
Prolume Ltd.
Rooke Agnes
Weber Jon
LandOfFree
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