Synthetic chimeric fimbrin peptides

Details Synthetic chimeric fimbrin peptides Synthetic chimeric fimbrin peptides

2007-06-12

2007-06-12

Duffy, Patricia A. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Amino acid sequence disclosed in whole or in part; or...

C424S192100, C424S197110

Reexamination Certificate

active

10223711

ABSTRACT:
The present invention provides synthetic chimeric fimbrin peptides which induce an immunogenic response in animals to non-typableHaemophilus influenzaeand that do not require tedious purification techniques. The synthetic chimeric fimbrin peptides reduce the severity of otitis media caused byHaemophilus influenzae. The synthetic chimeric fimbrin peptides are synthesized using commercially available peptide synthesizers. The synthetic chimeric fimbrin peptides comprises three peptide units. The first peptide unit is a subunit of the fimbrin protein. Preferably, the fimbrin subunit is comprised of the amino acids of Sequence ID No. 1 or Sequence ID No. 2. The second peptide unit is a t cell epitope, and preferably has the amino acid sequence of SEQ ID NO. 3. The third peptide unit is a linker peptide unit which joins the first and second peptide unit. The linking sequence preferably has from about 2 to about 15 amino acids, more preferably from about 2 to about 10 amino acids, most preferably from about 5 to about 6 amino acids. The synthetic chimeric fimbrin peptides are useful immunognes against NTHi and also useful as laboratory tool for detecting antibodies in sera. The invention also relates to an immunogenic composition containing the synthetic chimeric fimbrin peptides and a pharmacologically acceptable carrier.

REFERENCES:
patent: 5110908 (1992-05-01), Deich et al.
patent: 5766608 (1998-06-01), Kolattukudy et al.
patent: 5843464 (1998-12-01), Bakaletz et al.
patent: 6436405 (2002-08-01), Bakaletz et al.
patent: 94/26304 (1994-11-01), None
Sambrook et al (Molecular Cloning A Laboratory Manual, second edition), Cold Spring Harbor Laboratory Press, 1989, , vol. 3, chapter 17, pp. 17.1-17.44.
Rudinger et al, in “Peptide Hormones”, edited by Parsons, J.A., University Park Press, Jun. 1976, p. 6 (of record in IDS).
Burgess et al., The Journal of Cell Biology, 111:2129-2138, 1990 (of record in IDS).
Lazar et al., Molecular and Cellular Biology, 8(3):1247-1252, 1988 (of record in IDS).
Jobling et al. (Mol. Microbiol., 1991, 5(7):1755-67( of record in IDS).
“Possible Dissociation of the Heparin-binding and Mitogenic Activities of Heparin-binding (Acidic Fibroblast) Growth Factor-2 from Its Receptor-binding Activities by Site-Directed Mutagenesis of a Single Lysine Residue” by Burgess, et al.,The Journal of Cell Biology, vol. 111, 1990, pp. 2129-2138.
“Analysis of structure and function of the B subunit of cholera toxin by the use of site-directed mutagenesis” by Jobling, et al.,Molecular Microbiology, (1991)5(7) pp. 1755-1767.
“Characteristics of the amino acids as components of a peptide hormone sequence” by Rudinger, et al.,Peptide HormonesParson, J.A. editor, Baltimore University Park Press, 1976, pp. 1-6.
“Transforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities” by Lazar, et al.,Molecular and Cellular Biology, vol. 8, No. 3, Mar. 1998, pp. 1247-1252.
Abstract B-131 “Protection Against Histopathological Changes of Tympanic Membrane (TM) and Middle Ear Mucosa (MM) in a Chinchilla Model of Otitis Media by Immunization with Fimbrial Protein Isolated From NontypableHaemophilus influenza(NTHi) Strain #1128” by Bakaletz, et al., American Society for Microbiology, 93rd General Meeting, May 16-20, 1993, Atlanta, Georgia.
Abstract, “The Effect of Immunizations with Fimbrial Protein in a Chinchilla Model of NontypableHaemophilus influenzae-Induced Experimental Otitis Media” by Bakaletz, et al., Second Extraordinary International Symposium on Recent Advances in Otitis Media, Mar. 31-Apr. 3, 1993, Oita, Japan.
Abstract, “An Investigation of the Molecular Basis of the Adherence of NontypableHaemophilus influenzaeto Mucosal Epithelium” by Bakaletz, et al., Molecular Biology of Hearing and Deafness Conference, LaJalla, California, May 1-4, 1992.
Abstract B-169, “Passive Immunization of Chinchillas with Anti-NTHi Fimbrial Serum and Protection Against Experimental Otitis Media” by Bakaletz, et al. Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Serological Relatedness of Fimbriae Expressed by NTHi Isolates Recovered from Children with Chronic Otitis Media” by Bakaletz, et al., Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Isotype Specific Antibody Response Against OMPs and Fimbriae of NontypableHaemphilus influenzaeIsolated From Patients with Chronic Otitis Media” by DeMaria, et al., Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Immunological Responsiveness of Chincillas to Isolated Fimbrial Proteins of NontypableHemophilus influenzaeDuring Experimental Otitis Media” by Bakaletz, et al., Eleventh Midwinter Research Meeting, Association for Research in Otolaryngology, Jan. 31-Feb. 4, 1988, Clearwater Beach, Florida.
Abstract, “Inhibition of Adherence of NontypableHaemophilus influenzaeto Human Oropharyngeal Cells” by Bakaletz, et al., Twelfth Midwinter Research Meeting, Association for Research in Otolaryngology, Feb. 5-9, 1989, St. Petersburg Beach, Florida.
Abstract, “Role of Degree of Fimbriation on Ability of NontypableHaemophilus influenzaeto Colonize The Nasopharynx and Middle Ears of the Chinchilla” by Bakaletz, et al., Thirteenth Midwinter Research Meeting, Association for Research in Otolaryngology, Feb. 4-8, 1990, St. Petersburg Beach, Florida.
Abstract, Chinchilla Immunological Responsiveness to Isolated Outer Membrane and Fimbrial Proteins Of NontypableHaemophilus influenzae, by Bakaletz, et al., Thirteenth Midwinter Research Meeting, Association for Research in Otolaryngology, Feb. 4-8, 1990, St. Petersburg Beach, Florida.
Abstract B-66, Colonization of the Chinchilla Middle Ear and Nasopharynx by Fimbriated Isolates of NontypableHaemophilus influenzae, by Bakaletz, et al., 90th Annual Meeting of the American Society for Microbiology, May 13-17, 1990, Anaheim, California.
Abstract, “Presumptive Identification of the NTHi Adhesin for Human Oropharnygeal and Chinchilla Middle Ear Epithelial Cells” by Bakaletz, et al., Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Inhibition of Adherence of NTHi to Human Oropharyngeal Cells—An ELISA Essay” by Bakaletz, et al., Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Cloning and Sequencing of a Pilin Gene from NTHi Strain #1128” by Bakaletz, et al., Fifth International Symposium, Recent Advances in Otitis Media, May 20-24, 1991, Columbus, Ohio.
Abstract, “Protection of Chinchillas Against Experimental Otitis Media via Active Immunization with NTHi Strain #1128 Fimbrin” by Bakaletz, et al., Fifteenth Midwinter Research Meeting, Association for Research in Otolaryngology, Feb. 2-6, 1992, St. Petersburg Beach, Florida.
“Frequency of Fimbriate Isolates of NontypableHaemophilus influenzaefrom the Middle Ears and Nasopharynges of Patients with Chronic Otitis Meda” by Bakaletz, et al., Proceedings of the Fourth International Symposium, Recent Advances in Otitis Media, Jun. 1987, Columbus, Ohio, pp. 331-335.
“Evalution of pilus vaccines for prevention of experimental otitis media caused by nontypableHaemophilus influenzae” by Karasic, et al.,Pediatr Ifect Dis J, vol. 8, No. 1, 1989, pp. S62-S65.
“Cloning and Expression inEscherichia coliof LKP Pilus Genes from a NontypeableHaemophilus influenzaeStrain” by Kar, et al.,Infection and Immunity, vol. 58, No. 4, Apr. 1990, pp. 903-908.
“Design and Development of pilus vaccines forHaemophilus influenzaediseases” by Brinton, et al.,Pediatr Infect Dis J, vol. 8, No. 1, 1989, p. S54-S61.
Abstract, “Passive immunization of chinchi

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