Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1991-12-23
1999-08-03
LeGuyader, John L.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530300, 530387, 435 691, 43525233, C07K 1446
Patent
active
059326972
ABSTRACT:
A synthetic antifreeze peptide and a synthetic gene coding for the antifreeze peptide have been produced. The antifreeze peptide has a greater number of repeating amino acid sequences than is present in the native antifreeze peptides from winter flounder upon which the synthetic antifreeze peptide was modeled. Each repeating amino acid sequence has two polar amino acid residues which are spaced a controlled distance apart so that the antifreeze peptide may inhibit ice formation. The synthetic gene has been expressed in E. coli. A synthetic insert fragment has been prepared which can be readily inserted into the synthetic gene to alter the number of repeating units and/or amino acid composition in the antifreeze peptide produced.
REFERENCES:
patent: 4045910 (1977-09-01), Arny et al.
patent: 4161084 (1979-07-01), Arny et al.
patent: 4484409 (1984-11-01), Caple et al.
patent: 4601842 (1986-07-01), Caple et al.
patent: 4834899 (1989-05-01), Klenecz
patent: 4886663 (1989-12-01), Houghten
Duman, J.G. and A. L. DeVries. 1974. Freezing resistance in winter flounder Pseudopleronectes americanus Nature 247:237-238.
DeVries, A.L., and Y. Lin. 1977. Biochimica et Biophysica Acta 495:388-392. Structure of a Peptide Antifreeze and Mechanism of Adsorption to Ice.
Raymond, J. A., W. Radding, and A. L. DeVries. 1977. Biopolymers 16:2575-2578. Circular Dichroism of Protein and Glycoprotein Fish Antifreezes.
Raymond, J.A., and A.L. DrVries. 1977. Proceedings of the National Academy of Sciences of the USA 74:2589-2593. Adsorption inhibition as a mechanism of freezing resistance in polar fishes.
Lin, Y. and J.K. Gross. 1981. Proceedings of the National Academy of Sciences of the USA 78:2825-2829. Molecular cloning and characterization of winter flounder antifreeze cDNA.
Knight, C.A., A. L. DeVries, and L. K. Oolman. 1984, Nature 308:295-296. Fish antifreeze protein and the freezing and recrystallization of ice.
DeVries, A. L. 1984. Philosophical Transactions of the Royal Society of London (series B) 304:575-588. Role of glycopeptides and peptides in inhibition of crystallization of water in polar fishes.
Caple, G., W.L. Kerr, T.S. Burcham, D.T. Osuga, Y. Yeh, and R.E. Feeney. 1985. Journal of Colloid and Interface Science 111:299-304. Superadditive Effects in Mixtures of Fish Antifreeze Glycoptroteines and Polyalcohols or Surfactants.sup.1.2.
Hew, C.L., N.C. Wang, S.Yan, H.Cai, A.Sclater, and G.L. Fletcher. 1986. European Journal of Biochemistry 160:267-272. Biosysthesis of antifreeze polypeptides in the winter flounder.
Yang, D.S.C., Y.J. Chung, P. Chen, J.P. Rose, and D.L. Hew. 1986. Journal of Molecular Biology 189:725. Single Crystals of a Winter Flounder Antifreeze Polypeptide.
Bush, C.A. and R.E. Feeney, 1986. International Journal of Protein Research 28:386-397. Conformation of the glycoytripetide repeating unit of antifreeze glycoprotein of polar fish as determined from the fully assigned proton n.m.r. spectrum.
Scott, G.K., P.L. Davies, M.A. Shears, and G. L. Fletcher. 1987. European Journal of Biochemistry 168:629-633. Structural variations in the alanine-rich antifreeze proteins of the pleuronectinae.
Kao, M.H., G.L. Fletcher, N.C. Wang, and C.L.Hew 1986. Canadian Journal of Zoology 64:578-582. The relationship between molecular weight and antifreeze polypeptide activity in marine fish.sup.1.
Yang, D.S.C., M. Sax, A. Chakrabartty, and C.L. Hew. 1988. Nature 333:232 237 Crystal structure of an antifreeze polypeptide and its mechanistic implications.
Davies, P.L. C.L. Hew, and G.L. Fletcher, 1988, Canadian Journal of Zoology 66:2617-2622. Fish antifreeze proteins: physiology and evolutionary biology.
Parody-Morreale, A., K.P. Murphy, E.Di Cera, R. Fall, A.L. Devries, and S.J. Gill. 1989. Nature 333:782-783. Inhibition of bacterial ice nucleato by fish antifreeze glycoproteins.
Cutler, A.J., M. Saleem, E. Kendall, L.V. Gusta, F. Georges, and G.L. Fletcher. 1989. Journal of Plant Physiology 135:351-354. Winter Flounder Antifreeze Protein Improves the Cold Hardiness of Plant Tissues.
Chakrabartty, A., V.S. Ananthanarayanan, and C.L. Hew. 1989. Journal of Biological Chemistry 264:11307-11312. Structure-Function Relationship in a Winter Flounder Antifreeze Polypeptide
Chakrabartty, A., D.S.C. Yang, and C.L. Hew, 1989. Journal of Biological Chemistry 264:11313-11316. Structural Function Relationship in a Winter Flounder Antifreeze Polypeptide.
Knight, C.A. and A.L. DeVries. 1989. Science 245:505-507. Melting Inhibition and Superheating of Ice by Antifreeze Glycopeptide.
Raymond, J.A., P. Wilson, and A.L, Devries. 1989. Proceedings of the National Academy of Sciences of the USA 86:881-885. Inhibition of growth on nonbasal planes in ice by fish antifreezes.
Peters, I. D., C.L. Hew, and P.L. Davies, 1989. Protein Engineering 3:145-151. Biosysthesis of winter flounder antifreeze proprotein in E.coli.
Malcolm J. Casadaban, Joany Chou, and Stanley N. Cohen, 1980, In Vitro Gene Fusions That Join An Enzymatically Active B-Galactosidae Segment to Amino-Terminal Fragments of Exogenous Proteins: Escherichia coli Plasmid Vectors for the Detection and Cloning of Translational Initiation Signals; Journal of Bacteriology, p. 971, Stanford Univ. vol. 143, No. 2.
Joseph D. Schrag, Scott M. O'Grady and Arthur L. DeVries; Relationship of Amino Acid Composition and Molecular Weight of Antifreeze Glycopeptides to Non-Colligative Freezing Point Depression; 1982, Elsevier Biomedical Press, p. 322; Univ. of Illinois (No. 717).
F. William Studier & Barbara A. Moffatt: Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-Level Expression of Cloned Genes; 1986; J. Mol. Biol. p. 113-130; Brookhaven National Laboratory (No. 189).
Peter O. Olins, Catherine S. Devine, Shaukat H. Rangwala & Kamilla S. Kayka; The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli; 1988; Elsevier Science Publishers, pp. 227-235; Monsanto Company (No. 73).
Mark Pickett, Gary Scott, Peter Davies, Nam Wang, Shashikant Joshi and Choy Hew; 1984; Sequence of an antifreeze protein precursor; Eur. J. Biochem; pp. 35-38 (No. 143).
Gourlie et al, "Winter Flounder Antifreeze Proteins: A Multigene Family," The Journal of Biological Chemistry, vol. 259, No. 23, Dec. 10, 1984, pp. 14960-14965.
Burcham et al., "Antifreeze Glycoproteins: Influence of Polymer Length and Ice Crystal Habit on Activity," Biopolymers, vol. 23, 1379-1395 (1984).
Gourlic et al. J. Biol. Chem 259:14960 (1984).
Maniatis et al. Cloning Manual, CSH (1982).
Shen et al. PNAS (1984) 81:4627-4631.
Willson et al. Gene Anal. Tech. (1985) 2:7782.
Doel et al. NAR (1980) 8:4575-4592.
Kempe et al. Gene (1985) 39:239-245.
Caceci Thomas
Szumanski Maria B.
Toth Thomas E.
LeGuyader John L.
Virginia Tech Intellectual Properties Inc.
LandOfFree
Synthetic antifreeze peptide does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Synthetic antifreeze peptide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic antifreeze peptide will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-850430