Synthetic affinity ligand compositions and methods for purificat

Details Synthetic affinity ligand compositions and methods for purificat Synthetic affinity ligand compositions and methods for purificat

1989-09-08

1991-01-01

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435178, 435179, 435180, 435181, 435182, 435270, 435815, 436529, 436530, 436531, 436532, 436535, 546258, 530387, 530830, 530809, 530816, 536124, 536126, C12N 1106, C08F28300, A61K 39395

Patent

active

049819615

ABSTRACT:
A novel class of compounds, methods for the preparation thereof and the use thereof in chromatographic methods for binding various biologically active materials non-covalently are disclosed. The class of compounds comprises the reaction product of a polymeric gel with a pyridine base, such as 4-dimethylaminopyridine (DMAP), and a halogen-substituted pyridine, such as 3,5-dichloro-2,4,6-trifluoropyridine (DCTFP), which reaction product may in turn be optionally reacted with hydroxyl ions or specified low-molecular-weight compounds. These compounds are capable of selectively and efficiently binding proteins and other organic materials of interest non-covalently to a degree comparable or superior to the heretofore preferred natural affinity ligands, such as Protein A gels. The novel compounds find particular utility in purification and recovery of proteins such as serum albumin and immunoglobulins of various classes from crude sources, such as diluted serum samples from various species.

REFERENCES:
patent: 4415665 (1983-11-01), Mosbach et al.
patent: 4582875 (1986-04-01), Ngo
patent: 4704366 (1987-11-01), Juarez-Salinas et al.
patent: 4801687 (1989-01-01), Ngo
patent: 4886755 (1989-12-01), Ngo

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