Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2002-11-18
2010-02-16
Lu, Frank W (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S320100, C536S023100, C536S024300, C536S024330
Reexamination Certificate
active
07662551
ABSTRACT:
A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).
REFERENCES:
patent: 4683202 (1987-07-01), Mullis et al.
patent: 4888286 (1989-12-01), Crea
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4994368 (1991-02-01), Goodman et al.
patent: 5023171 (1991-06-01), Ho et al.
patent: 5043272 (1991-08-01), Hartley
patent: 5223408 (1993-06-01), Goeddel et al.
patent: 5252479 (1993-10-01), Srivastava
patent: 5270170 (1993-12-01), Schatz et al.
patent: 5338665 (1994-08-01), Schatz et al.
patent: 5491074 (1996-02-01), Aldwin et al.
patent: 5498530 (1996-03-01), Schatz et al.
patent: 5502167 (1996-03-01), Waldmann et al.
patent: 5512463 (1996-04-01), Stemmer
patent: 5514568 (1996-05-01), Stemmer
patent: 5521291 (1996-05-01), Curiel et al.
patent: 5573907 (1996-11-01), Carrino et al.
patent: 5605793 (1997-02-01), Stemmer
patent: 5712089 (1998-01-01), Borrebaeck et al.
patent: 5714316 (1998-02-01), Weiner et al.
patent: 5723323 (1998-03-01), Kauffman et al.
patent: 5733731 (1998-03-01), Schatz et al.
patent: 5733753 (1998-03-01), Jørgensen
patent: 5763192 (1998-06-01), Kauffman et al.
patent: 5798208 (1998-08-01), Crea
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5814476 (1998-09-01), Kauffman et al.
patent: 5817483 (1998-10-01), Kauffman et al.
patent: 5824514 (1998-10-01), Kauffman et al.
patent: 5830650 (1998-11-01), Crea
patent: 5830696 (1998-11-01), Short
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5834252 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 5858657 (1999-01-01), Winter et al.
patent: 5858725 (1999-01-01), Crowe et al.
patent: 5871907 (1999-02-01), Winter et al.
patent: 5925544 (1999-07-01), Jørgensen
patent: 5928905 (1999-07-01), Stemmer et al.
patent: 5939250 (1999-08-01), Short
patent: 5965408 (1999-10-01), Short
patent: 5969108 (1999-10-01), McCafferty et al.
patent: 5976862 (1999-11-01), Kauffman et al.
patent: 6096548 (2000-08-01), Stemmer
patent: 6117679 (2000-09-01), Stemmer
patent: 6132970 (2000-10-01), Stemmer
patent: 6143527 (2000-11-01), Pachuk et al.
patent: 6153410 (2000-11-01), Arnold et al.
patent: 6156511 (2000-12-01), Schatz et al.
patent: 6159687 (2000-12-01), Vind
patent: 6159688 (2000-12-01), Borchert et al.
patent: 6159690 (2000-12-01), Borrebaeck et al.
patent: 6165793 (2000-12-01), Stemmer
patent: 6177263 (2001-01-01), Arnold et al.
patent: 6180406 (2001-01-01), Stemmer
patent: 6355484 (2002-03-01), Patten et al.
patent: 6368861 (2002-04-01), Crameri et al.
patent: 6413774 (2002-07-01), Stemmer et al.
patent: 2004/0048268 (2004-03-01), Delcourt et al.
patent: 0 368 684 (1990-05-01), None
patent: 0 415 731 (1991-03-01), None
patent: 0415731 (1991-03-01), None
patent: 0 456 304 (1991-11-01), None
patent: 0557897 (1993-09-01), None
patent: 0 590 689 (1994-04-01), None
patent: 0911396 (1999-04-01), None
patent: 0552266 (2000-01-01), None
patent: 2813314 (2002-03-01), None
patent: 9712512.4 (1997-06-01), None
patent: 10-66576 (1998-03-01), None
patent: 2000-308490 (2001-11-01), None
patent: WO 86/05803 (1986-10-01), None
patent: 9007936 (1990-07-01), None
patent: WO 90/07936 (1990-07-01), None
patent: 9014430 (1990-11-01), None
patent: WO 90/14430 (1990-11-01), None
patent: WO 91/07506 (1991-05-01), None
patent: 9116427 (1991-10-01), None
patent: WO 91/15581 (1991-10-01), None
patent: WO 91/16427 (1991-10-01), None
patent: WO 92/01047 (1992-01-01), None
patent: 9207075 (1992-04-01), None
patent: WO 92/07075 (1992-04-01), None
patent: WO 92/15702 (1992-09-01), None
patent: WO 92/18645 (1992-10-01), None
patent: 9302191 (1993-02-01), None
patent: 9307282 (1993-04-01), None
patent: WO 93/06213 (1993-04-01), None
patent: WO 93/07282 (1993-04-01), None
patent: WO 93/08278 (1993-04-01), None
patent: 9311237 (1993-06-01), None
patent: WO 93/12257 (1993-06-01), None
patent: 9315208 (1993-08-01), None
patent: 9316192 (1993-08-01), None
patent: 9319172 (1993-09-01), None
patent: 9409817 (1994-05-01), None
patent: 9413804 (1994-06-01), None
patent: WO 94/12632 (1994-06-01), None
patent: WO 94/24313 (1994-10-01), None
patent: WO 94/28173 (1994-12-01), None
patent: WO 95/22625 (1995-08-01), None
patent: WO 96/05803 (1996-02-01), None
patent: WO 96/17056 (1996-06-01), None
patent: WO 96/40750 (1996-12-01), None
patent: WO 96/40987 (1996-12-01), None
patent: WO 97/07205 (1997-02-01), None
patent: WO 97/07206 (1997-02-01), None
patent: WO 97/08320 (1997-03-01), None
patent: 9720078 (1997-06-01), None
patent: WO 97/20078 (1997-06-01), None
patent: WO 97/35957 (1997-10-01), None
patent: WO 97/35966 (1997-10-01), None
patent: WO 98/01581 (1998-01-01), None
patent: WO 98/05765 (1998-02-01), None
patent: WO 98/13485 (1998-04-01), None
patent: WO 98/13487 (1998-04-01), None
patent: WO 98/25965 (1998-06-01), None
patent: WO 98/27230 (1998-06-01), None
patent: 9832845 (1998-07-01), None
patent: WO 98/28416 (1998-07-01), None
patent: WO 98/31837 (1998-07-01), None
patent: WO 98/32845 (1998-07-01), None
patent: WO 98/41622 (1998-09-01), None
patent: WO 98/41623 (1998-09-01), None
patent: WO 98/41653 (1998-09-01), None
patent: WO 98/42832 (1998-10-01), None
patent: 9858080 (1998-12-01), None
patent: WO 98/58080 (1998-12-01), None
patent: WO 99/21979 (1999-05-01), None
patent: WO 99/23107 (1999-05-01), None
patent: WO 99/23236 (1999-05-01), None
patent: WO 99/33965 (1999-07-01), None
patent: WO 99/41368 (1999-08-01), None
patent: WO 99/41369 (1999-08-01), None
patent: WO 99/41383 (1999-08-01), None
patent: WO 99/41402 (1999-08-01), None
patent: WO 99/45110 (1999-09-01), None
patent: WO 99/57128 (1999-11-01), None
patent: WO 99/58661 (1999-11-01), None
patent: WO 99/65927 (1999-12-01), None
patent: WO 00/04190 (2000-01-01), None
patent: WO 00/09679 (2000-02-01), None
patent: WO 00/09727 (2000-02-01), None
patent: WO 00/12680 (2000-03-01), None
patent: WO 00/18906 (2000-04-01), None
patent: WO 00/20573 (2000-04-01), None
patent: WO 00/28008 (2000-05-01), None
patent: WO 00/28017 (2000-05-01), None
patent: WO 00/28018 (2000-05-01), None
patent: WO 00/34512 (2000-06-01), None
patent: WO 00/42559 (2000-07-01), None
patent: WO 00/42560 (2000-07-01), None
patent: WO 00/42561 (2000-07-01), None
patent: WO 00/46344 (2000-08-01), None
patent: WO 00/52155 (2000-09-01), None
patent: WO 00/53744 (2000-09-01), None
patent: WO 00/58517 (2000-10-01), None
patent: WO 00/61731 (2000-10-01), None
patent: WO 00/61740 (2000-10-01), None
patent: WO 00/72013 (2000-11-01), None
patent: WO 00/73433 (2000-12-01), None
Attached one of definitions for “cloning”.
Alber, Tom, et al., “Contributions of hydrogen bonds of Thr 157 to the thermodynamic stability of phage T4 lysozyme.” Nature, 330: 41-46 (1987).
Arrizubieta, Maria Jesus and Julio Polaina. “Increased Thermal Resistance and Modification of the Catalytic Properties of a β-Glucosidase by Random Mutagenesis and in Vitro Recombination.” The Journal of Biological Chemistry, vol. 275, No. 37: 28843-28848 (2000).
Barbas, III, Carlos F., et al., “Assembly of combinatorial antibody libraries on phage surfaces: The gene III site.” Proc. Natl. Acad. Sci., 88: 7978-7982 (1991).
Barbas, III, Carlos F., et al., “Semisynthetic combinatorial antib
Harayama Shigeaki
Kikuchi Miho
Ohnishi Kouhei
Alligator Bioscience AB
Dann Dorfman Herrell & Skillman
Lu Frank W
Netter Robert C.
LandOfFree
Synthesis of hybrid polynucleotide molecules using... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Synthesis of hybrid polynucleotide molecules using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthesis of hybrid polynucleotide molecules using... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4167483