Drug – bio-affecting and body treating compositions – Lymphokine
Patent
1985-12-12
1988-09-06
Tarcza, John E.
Drug, bio-affecting and body treating compositions
Lymphokine
435 70, 4351723, 435256, 530350, 530806, 530824, 530826, 935 12, 935 28, 935 37, 935 69, A61K 3929, C12P 2102, C12N 1500, C12N 118
Patent
active
047692383
ABSTRACT:
The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADHl, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector.
Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBsAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
REFERENCES:
patent: 4181713 (1980-01-01), McAleer et al.
patent: 4547367 (1985-10-01), Tabor et al.
patent: 4547368 (1985-10-01), Tabor et al.
Cabral et al, Journal of General Virology, 38, pp. 339-350 (1978).
Skelly et al, Nature, 290, pp. 51-54 (1981).
Edman et al, Nature, 286, pp. 535-538 (1980).
Dubois et al, Proc. Natl. Acad. Sci., 77(8), pp. 4549-4553 (1980).
Hitzeman et al, Nature, 293, pp. 717-722 (1981).
Fraser et al, "Synthesis of Chicken Ovalbumin in Saccharomyces Cerevisiae", Microbiology--1981, ed. Schlessinger, A.S.M., pp. 392-395 (1981).
Ammerer Gustav
Hall Benjamin D.
Rutter William J.
Valenzuela Pablo D. T.
Tarcza John E.
The Regents of the University of California
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