Synthesis of full-length, double-stranded DNA from a single-stra

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state... – Using fungi

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 6, 435 913, 435911, C12P 1934, C12Q 168

Patent

active

056437668

ABSTRACT:
A method for preparing a ds DNA from a ss DNA template, which method includes:

REFERENCES:
patent: 4661450 (1987-04-01), Kempe et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4985359 (1991-01-01), Oberbaumer
patent: 4987073 (1991-01-01), Berman et al.
patent: 5023243 (1991-06-01), Tullis
patent: 5162209 (1992-11-01), Scheele
patent: 5437990 (1995-08-01), Burg et al.
Fritz et al, "A novel 3' extension technique using random primers in RNA-PCR", Nucleic Acids Res. 19(13):3747 1991.
Pullen et al, "Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase", J. Virol. 66(1):367-373 Jan. 1992.
Weiss et al, "Synthetic human tRNAlys3 and natural bovine tRNAlys3 interact with HIV reverse transcriptase and serve as specific primers for retroviral cDNA synthesis", Gene 111:183-197 Feb. 1992.
Land et al, (1981), "5' terminal sequences of eukcaryotic mRNA can be cloned with high efficiency", Nucleic Acids Res. 9(10):2251-2266.
Xu et al, (1987), "Tailored removal of flanking homopolymer sequences from cDNA clones", DNA 6(5):505-513.
Yoon et al, (1989), "A Simple and versatile method for the preparation of vector-primers by adapter-end-primer ligation", Biotechniques 7(9):992-997.
Asamizu et al, (1985), "Molecular cloning and characterization of the genome of Wound Tumor Virus: A tumor inducing plant reovirus", Virology 144:398-409.
Been and Cech, RNA as an RNA Polymerase: Net Elongation of an RNA Primer Catalyzed by the Tetrahymena Ribozyme, Science 239:1412-1416, 1988.
Boehringer Mannheim Biochemicals for Molecular Biology Catalog 1990, p. 144.
Gaubatz and Paddock, Strategies for Constructing Complementary DNA for Cloning J. Theor. Biol. 95:679-696, 1982.
Gubler and Hoffman, A Simple and Very Efficient Method for Generating cDNA Libraries, Gene 25:263-269, 1983.
Kochetkov et al., Organic Chemistry of Nucleic Acids, Part A, Kochetkov and Budovskii eds., Plenum Press (London and New York, 1971), pp. 81-83.
Land et al., 5'-Terminal Sequences of Eukaryotic mRNA can be Cloned with High Efficiency Nucleic Acids Research 9:2251-2266, 1981.
Ogilvie et al., Synthesis of Oligoribonucleotides, J. Am. Chem. Soc. 99:7741-7743, 1977.
Okayama and Berg, High-Efficiency Cloning of Full-Length cDNA, Molecular and Cellular Biology 2:161-170, 1982.
PCT Search Report for the corresponding PCT Applications No. PCT/US92/00363.
PCT Written Opinion for the corresponding PCT application No. PCT/US92/00363.
Schubert et al., Primary Structure of the Vesicular Stomatitis Virus Polymerase (L) Gene: Evidence for a High Frequency of Mutations, J. Virology 51:505-514, 1984.
Stratagene Cloning Systems 1990 Product Catalog, pp. 58-59.
Strub et al., The cDNA Sequences of the Sea Urchin U7 Small Nuclear RNA Suggest Specific Contacts Between Histone mRNA Precursor and U7 RNA During RNA Processing, EMBO Journal 3:2801-2807.
Usman et al., Preparation of Ribonucleoside 3' -o-phosphoramidites and Their Application to the Automated Solid Phase Synthesis of Oligonucleotides, Tetrahedron Letters 26:4567-4570, 1985.
Usman et al., Automated Chemical Synthesis of Long Oligoribonucleotides Using 2'-O-Silylated Ribonucleoside . . . Molecule of the Escherichia coli Formylmethionne tRNA, J. American Chemical Society 109:7845-7854, 1987.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Synthesis of full-length, double-stranded DNA from a single-stra does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthesis of full-length, double-stranded DNA from a single-stra, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthesis of full-length, double-stranded DNA from a single-stra will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-596047

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.