Synthesis of DNA

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100

Reexamination Certificate

active

07452666

ABSTRACT:
A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

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Stemmer et al. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene, vol. 164, p. 49-53, 1995.
Heller et al. Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and other genomic applications. Electrophoresis, vol. 21, pp. 157-164, 2000.
Stemmer, W., et al., “Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides,” Elsevier Science B.V., Gene, 164 (1995) pp. 49-53.
Heller, M. J., et al., “Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and other genomic applications,” Electrophoresis 2000, 21, Wiley-VCH Verlat GmbH, 69451 Weinheim, (2000) pp. 157-164.

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