Synthesis of diverse and useful collections of oligonucleotidies

Details Synthesis of diverse and useful collections of oligonucleotidies Synthesis of diverse and useful collections of oligonucleotidies

1996-08-08

1999-02-09

Chambers, Jasemine C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4351723, 435 911, C07H 2100, C12N 1500

Patent

active

058696440

ABSTRACT:
A new technique for generating mixtures of oligonucleotides in a single automated synthesis is taught. The method can be used to prepare mixed oligonucleotides ideally suited for creation of useful mixtures of oligo- or polypeptides or proteins. Additionally, the technique enables insertion and/or substitution and/or deletion of a nucleotide sequence at one or more sites. For protein mutagenesis, a trinucleotide can be inserted or substituted at codon boundaries. The invented technique makes possible the encoding of all possible single amino acid insertions, or any desired mixture of substitutions and insertions.

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