Synthesis of complex polyamines for ampholyte production

Details Synthesis of complex polyamines for ampholyte production Synthesis of complex polyamines for ampholyte production

1991-02-27

1994-06-21

Kight, III, John

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525 541, 525 5411, 525421, 528229, 528324, 528332, 5283395, 560171, 560190, 560205, 2041801, 2041832, C08G 6348, C08G 6391

Patent

active

053229061

ABSTRACT:
New synthesis and analysis procedures for novel crosslinked polyamines and ampholytes. Polyamines are crosslinked with tartaric acid ester, malonic acid ester, or polycarboxylic acid esters of the citric acid cycle. The resulting crosslinked polyamine may further react with other compounds such as an .alpha.,.beta.-unsaturated or .alpha.-halo unsaturated carboxylic acids to prepare new ampholyte mixtures. The resulting novel ampholytes exhibit greater heterogeneity and complexity than presently prepared ampholytes, and can be used in analytical and preparative isoelectric focusing processes.
Novel ampholyte analysis process entails analyzing chemical compounds, usually ampholytes, used in isoelectric focusing processes. The ampholyte is isoelectrically focused on an immobilized pH gradient, and then immersed in a picric acid solution to cause precipitation and visualization of amphoteric species. The isoelectrically focused and visualized ampholyte compounds may be photographed or analyzed densitometrically to compare it with other isoelectrically focused and visualized ampholytes to determine relative heterogeneity of the ampholytes over given pH ranges.

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