Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1993-09-16
1997-05-13
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435170, 4351723, C12P 722, C12P 104, C12N 1509
Patent
active
056291817
ABSTRACT:
A method is provided for synthesizing catechol from a biomass-derived carbon source capable of being used as a host cell having a common pathway of aromatic amino acid biosynthesis. The method comprises the steps of biocatalytically converting the carbon source to 3-dehydroshikimate in said host cell, biocatalytically converting the DHS to protocatechuate, and decarboxylating the protocatechuate to form catechol. Also provided is a heterologous E. coli transformant characterized by the expression of genes encoding transhetolase, DAHP synthase, and DHQ synthase, further characterized by the constitutive expression of structural genes encoding 3-dehydroshikimate dehydratase and protocatechuate decarboxylase.
REFERENCES:
patent: 3970522 (1976-07-01), Sasajima et al.
patent: 4681852 (1987-07-01), Tribe
patent: 4753883 (1988-06-01), Backman et al.
patent: 4908312 (1990-03-01), Ozaki et al.
patent: 5017481 (1991-05-01), Matsui et al.
patent: 5168056 (1992-12-01), Frost
patent: 5272073 (1993-12-01), Frost et al.
Bailey (1991) Science, vol. 252, pp. 1668-1675.
A.J. Pittard, "Biosynthesis of the Aromatic Amino Acids", in Escherichia coli & Salmonella typhimurium, Editor in Chief: F.C. Neidhardt, American Soc. for Microbiology, Washington, pp. 368-394, 1987.
K.M. Herrmann et al., "The Common Aromatic Biosynthetic Pathway" in Amino Acids: Biosynthesis and Genetic Regulation, Addison-Wesley Reading, pp. 301-376, 1983.
K.M. Draths et al. "Synthesis Using Plasmid-Based Biocatalysis: Plasmid Assembly and 3-Deoxy-arabino-heptulosonate Production", JACS, vol. 112, pp. 1657-1659, 1990.
K.M. Draths & J.S. Frost, "Genomic Direction of Synthesis During Plasmid-Based Biocatalysis", JACS, 1990, V. 112, p. 9630.
Stu Borman, "New biosynthehtic route to catechol discovered," C&EN, p. 26, Jan. 6, 1992.
K.M. Draths and J.W. Frost, "Conversion of D-Glucose into Catehol: The Not-So-Common Pathway of Aromatic Biosynthesis", J. Am. Chem. Soc., vol. 113, No. 25, pp. 9361-9363, 1991.
David Brewster, Robin S. Jones and Dennis V. Parke, "Aromatization of Shikimic Acid in the Rat and the Role of Gastrointestinal Micro-Organisms", 562nd Meeting of Biochemical Society Transactions; pp. 518-521,1976.
David Brewster, Robin S. Jones and Dennis V. Parke, "The Metabolism of Shikimate in the Rat", Biochem J. vol. 170, pp. 257-264, 1978.
Neil C. Bruce and Ronald B. Cain, "Hydroaromatic Metabolism in Rhodococcus Rhodochrous: Purification and Characterisation of its NAD-dependent Quinate Dehydrogenase", Archives of Microbiology, vol. 154 pp. 179-186, 1990.
John W. Frost, Judith L. Bender, James T. Kadonaga and Jeremy R. Knowles, "Dehydroquinate Synthase from Escherichia coli: Purification, Cloning, and Construction of Overproducers of the Enzyme", Biochemistry, vol. 23, pp. 4470-4475, 1984.
K.M. Draths and J.W. Frost, "Genomic Direction of Synthesis during Plasmid-Based Biocatalysis", Journal of the American Chemical Society, vol. 112, pp. 9630-9632, 1990.
J.L. Canovas, M.L. Whellis, and R.Y. Stanier, "Regulation of the Enzymes of the .beta.-Ketodipate Pathway in Moraxella calcoacetica--The Role of Protocatechuate as Inducer", European Journal of Biochemistry, vol. 3, pp. 293-304, 1968.
W.M. Ingledew, M. Elena F. Tresguerres and J.L. Canovas, "Regulation of the Enzymes of the Hydroaromatic Pathway in Acinetobacter calco-aceticus", The Journal of General Microbiology, vol. 68, pp. 273-282, 1971.
J. Elena F. Tresguerres, W.M. Ingledew, and J.L. Canovas, "Potential Competition for 5-Dehydroshikimate between the Aromatic Biosynthetic Route and the Catabolic Hydroaromatic Pathway", Arch. Mikrobiol., vol. 82, pp. 111-119, 1972.
Heather K. Lamb, Clive R. Bagshaw, and Alastair R. Hawkins, "In vivo overproduction of the pentafunctional arom polypeptide in Aspergillus nidulans affects metabolic flux in the quinate pathway", MGG, vol. 227, pp. 187-196, 1991.
R.B. Cain, "The Regulation of Enzymes of Aromatic-Ring Fission in Fungi: Organisms using only the Protocatechuate Pathway", Biochemical Journal vol. 114(4) p. 76, 1969.
R.S. Chaleff, "The Inducible Quinate-Shikimate Catabolic Pathway in Neurospora Crassa: Genetic Organization", Journal of General Microbiology, vol. 81 pp. 337-355, 1974.
C. Ratledge, "The Production of an N-Acylanthranilic Acid From Shikimic Acid and the Effect of Iron Deficiency on the Biosynthesis of other Aromatic Compounds By Aerobacter aerogenes", Biochimica Et Biophysica Acta, vol. 141 pp. 55-63, 1967.
J.C. Patel and D.J.W. Grant, "The Formation of Phenol in the Degradation of p-hydroxybenzoic acid by Klebsiella aerogenes (Aerobacter aerogenes)", Antonie van Leeuwenhoek, vol. 35, pp. 53-64, 1969.
A.J. Pittard, F. Gibson and C.H. Doy, "A Possible Relationship Between The Formation of o-Dihydric Phenols and Tryptophan Biosynthesis by Aerobactor aerogenes", Biochimica ET Biophysica Acta, vol. 57, pp. 290-298, 1962.
A.J. Pittard, F. Gibson and C.H. Doy, "Phenolic Compounds Accumulated By Washed Cell Suspensions of a Tryptophan Auxotroph of Aerobacter aerogenes", Biochim, Biophys, Acta, vol. 49, pp. 485-494, 1961.
D.J.W. Grant, "The Oxidative Degradation of Benzoate and Catechol by Klebsiella aerogenes (Aerobacter aerogenes)", Antonie van Leeuwenhock, vol. 36, pp. 161-177, 1970.
Katsuhisa Shirai, "Catechol Production from Benzene through Reaction with Resting and Immobilized Cells of Mutant Strain of Pseudomonas", Agric. Biol. Chem., vol. 51(1), pp. 121-128, 1987.
Katsuhisa Shirai, "Screening of Microorganisms for Catechol Production from Benzene", Agric. Biol. Chem., vol. 50(11) pp. 2875-2880, 1986.
Draths Karen M.
Frost John W.
Carter Philip W.
Fleisher Mindy
Purdue Research Foundation
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