Synthesis-deficient thermostable DNA polymerase

Details Synthesis-deficient thermostable DNA polymerase Synthesis-deficient thermostable DNA polymerase

1995-06-06

1998-08-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435199, 435 6, C12N 912, C12N 922, C12Q 168

Patent

active

057957630

ABSTRACT:
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis et al.
patent: 5108892 (1992-04-01), Burke et al.
patent: 5144019 (1992-09-01), Rossi
patent: 5210015 (1993-05-01), Gelfand et al.
J. Marmur and D. Lane, "Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Biological Studies," Proc. Natl. Acad. Sci. USA 46:453 (1960).
P. Doty et al., "Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Physical Chemical Studies," Proc. Natl. Acad. Sci. USA 46:461 (1960).
M. Hayashi et al., "Restriction of In Vivo Genetic Transcription to One of the Complementary Strands of DNA," Proc. Natl. Acad. Sci. USA 50:664 (1963).
H. O. Smith and K. W. Wilcox, "A Restriction Enzyme from Hemophilus influenza," J. Mol. Biol. 51:379 (1970).
E. M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis," J. Mol. Biol. 98:503 (1975).
R. B. Wallace et al., "Application of Synthetic Oligonucleotides to the Diagnosis of Human Genetic Diseases," Biochimie 67:755 (1985).
A. B. Studencki and R. B. Wallace, "Allele-Specific Hybridization Using Oligonucleotide Probes of Very High Specific Activity: Discrimination of the Human .beta..sup.A -and .beta..sup.S -Globin Genes," DNA 3:7 (1984).
R.K. Saiki, PCR Technology--Principles and Application for DNA Amplification(H.A. Erlich, Ed.), Stockton Press, New York, pp. 7-19 (1989).
A.B. Studencki et al., "Allele-Specific Hybridization Using Oligonucleotide Probes of Very High Specific Activity: Discrimination of the Human .beta.A-and .beta.S-Globin Genes," Am. J. Human Genetics 37:42 (1985).
P. M. Holland et al., "Detection of Specific Polymerase Chain Reaction Product by Utilizing the 5'-3' Exonuclease Activity of Thermus aquaticusDNA Polymerase," Proc. Natl. Acad. Sci. USA 88:7276 (1991).
R. B. Wallace et al., "Hybridization of Synthetic Oligodeoxyribonucleotides to .PHI..sub.X 174 DNA: the Effect of Single Base Pair Mismatch," Nucl. Acids Res. 6:3543 (1979).
R. B. Wallace et al., "The Use of Oligonucleotides as Hybridization Probes. II. Hybridization of Oligonucleotides of Mixed Sequence to Rabbit .beta.-Globin DNA," Nucl. Acids Res. 9:879 (1981).
R. K. Saiki et al., "Analysis of Enzymatically Amplified .beta.-Globin and HLA-DQ.alpha.DNA with Allele-Specific Oligonucleotide Probes," Nature 324:163 (1986).
S. Kwok et al., "Effects of Primer-Template Mismatches on the Polymerase Chain Reaction: Human Immunodeficiency Virus Type 1 Model Studies," Nucl. Acids Res. 18:999 (1990).
A. Kornberg, DNA Replication, W.H. Freeman and Co., San Francisco, pp. 127-139 (1980).
K.R. Tindall and T.A. Kunkel, "Fidelity of DNA Synthesis by the Thermus aquaticusDNA Polymerase" Biochem. 27:6008 (1988).
D. Brutlag et al., "An Active Fragment of DNA Polymerase Produced by Proteolytic Cleavage," Biochem. Biophys. Res. Commun. 37:982 (1969).
H.A. Erlich et al., "Recent Advances in the Polymerase Chain Reaction," Science 252:1643 (1991).
P. Setlow and A. Kornberg, "Deoxyribonucleic Acid Polymerase: Two Distinct Enzymes in One Polypeptide--II.," J. Biol. Chem. 247:232 (1972).
D.H. Gelfand, PCR Technology--Principles and Applications for DNA Amplification(H.A. Erlich, Ed.), Stockton Press, New York, p. 19 (1989).
F.C. Lawyer et al., "Isolation, Characterization, and Expression in Escherichia coliof the DNA Polymerase Gene from Thermus aquaticus," J. Biol. Chem. 264:6427 (1989).
A.A. Akhmetzjanov and V.A. Vakhitov, "Molecular Cloning and Nucleotide Sequence of the DNA Polymerase Gene from Thermus flavus," Nucl. Acids Res. 20:5839 (1992).
P. Setlow et al., "Deoxyribonucleic Acid Polymerase: Two Distinct Enzymes in One Polypeptide--I.," J. Biol. Chem. 247:224 (1972).
R.K. Saiki et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase," Science 239:487 (1988).
K.B. Mullis and F.A. Faloona, "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction," Methods in Enzymology155:335 (1987).
F.B. Perler et al., "Intervening Sequences in an Archaea DNA Polymerase Gene," Proc. Natl. Acad. Sci. USA 89:5577 (1992).
A.S.Kaledin et al., "Isolation and Properties of DNA Polymerase from the Extremely Thermophilic Bacterium Thermus flavus," Biokhimiya 46:1576 (1981).
N. Carballeira et al., "Purification of a Thermostable DNA Polymerase from Thermus thermophilusHB8, Useful in the Polymerase Chain Reaction," BioTechniques 9:276 (1990).
T.W. Myers and D.H. Gelfand, "Reverse Transcription and DNA Amplification by a Thermus thermophilusDNA Polymerase," Biochem. 30:7661 (1991).
J. Ito and D.K. Braithwaite, "Compilation and Alignment of DNA Polymerase Sequences," Nucl. Acids Res. 19:4045 (1991).
E.J. Mathur et al., "The DNA Polymerase Gene from the Hyperthermophilic Marine Archaebacterium, Pyrococus furiosus,Shows Sequence Homology with .alpha.-like DNA Polymerases," Nucl. Acids Res. 19:6952 (1991).
J.J. Dunn and F. W. Studier, "Complete Nucleotide Sequence of Bacteriophage T7 DNA and the Locations of T7 Genetic Elements," J. Mol. Biol. 166:477 (1983).
V.P. Antao et al., "A Thermodynamic Study of Unusually Stable RNA and DNA Hairpins," Nucl. Acids Res. 19:5901 (1991).
M.J.R. Stark, "Multicopy Expression Vectors Carrying the lac Repressor Gene for Regulated High-Level Expression of Genes in Escherichia coli," Gene 51:255 (1987).
F.W. Studier and B.A. Moffatt, "Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-Level Expression of Cloned Genes," J. Mol. Biol. 189-113 (1986).
J. Sambrook, et al., Molecular Cloning,Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp. 1.63-1.69 (1989).
D.R. Englke et al., "Purification of Thermus aquaticusDNA Polymerase Expressed in Escherichia coli," Anal. Biochem. 191:396 (1990).
A. Kornberg, et al., DNA Replication (2nd ed.) W.H. Freeman and Co., San Francisco, pp. 403-414 (1992).
M. Orita et al., "Rapid and Simple Detection of Point Mutations and DNA Polymorphisms Using the Polymerase Chain Reaction," Genomics 5:874 (1989).
K. Hayaski, "PCR-SSCP: A Simple and Sensistive Method for Detection of Mutations in the Genomic DNA," PCR Meth. and Appl. 1:34 (1991).
M. Bargseid, et al., "A High Fidelity Thermostable DNA Polymerase Isolated from Pyrococcus furiosus," Strategies (Stratagene, LaJolla, CA) 4:34 (1991).
R.B. Kelley, et al., "Excision of Thymine Dimers and Other Mismatched Sequences by DNA Polymerase of Escherichia coli,"Nature 244:495 (1969).
A. Kornberg et al., "Enzymatic Synthesis of Deoxyribonucleic Acid, XVL Oligonucleotides as templates and the Mechanism of Their Replication," Biochemistry 51:315 (1961).
A. Kornberg, "DNA Polymerases--A Perspective," The Enzymes, vol. XIV:3 (1981).
I.R. Lehman, "DNA Polymerase I of Escherichia coli," The Enzymes, vol. XIV:15 (1981).
P. Lopez et al., "Characterization of the polA Gene of Strepococcus pneumoniaeand Comparision of the DNA Polymerase I It Encodes to Homologous Enzymes from Escherichia coliand Phage T7," J. Biol. Chem. 264:4255 (1989).
H.K. Schachman, et al., "Enzymatic Synthesis of Deoxyribonucleic Acid," J. Biol. Chem. 235:3242 (1960).
R.C. Lundquist, et al., "Transient Generation of Displaced Single-Stranded DNA During Nick Translation," Cell 31:53 (1982).
M.A. Innis, et al., "DNA Sequencing with Thermus aquaticusDNA Polymerase and Direct Sequencing of Polymerase Chain Reaction-Amplified DNA," Proc. Natl. Acad. Sci. USA 85:9436 (1988).
Promega, Product Analysis: "Taq DNA Polymerase", Certificate of Analysis (1990).
M.J. Longley et al., "Characterization of the 5'to 3' Exonuclease Associated with Thermus aquaticusDNA Polymerase," Nucl. Acids Res. 18:7317 (1990).
Y. Li et al., "Targeted Cleavage of mRNA in vitro by RNase P from Escherichia coli," Proc. Natl. Acad. Sci. USA 89:3185 (1992).
A.J. Podhajska et al., "Conversion of the FokI Endonuclease to a Universal Restri

Affiliated with

Also associated with

Rating

Synthesis-deficient thermostable DNA polymerase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthesis-deficient thermostable DNA polymerase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthesis-deficient thermostable DNA polymerase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1113961