Synthesis and screening of immobilized oligonucleotide arrays

Details Synthesis and screening of immobilized oligonucleotide arrays Synthesis and screening of immobilized oligonucleotide arrays

1992-09-30

1996-04-23

Spiegel, Carol A.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 4, 435 6, 435 792, 435968, 435969, 435973, 436527, 436530, 436531, 436532, 422131, 422134, 536 253, 536 2531, 536 2532, 536 2533, 536 2534, 935 88, G01N 3353

Patent

active

055102700

ABSTRACT:
A method for synthesizing and screening oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of a substrate comprising immobilized nucleotides on its surface, without irradiation of a second predefined region of the substrate. The irradiation step removes a protecting group from the immobilized nucleotides. The substrate is contacted with a first nucleotide to couple the nucleotide to the immobilized nucleotides in the first predefined region without coupling in the second predefined region. At least a part of the first predefined region and at least a part of the second predefined region are subjected to further irradiation. The substrate is contacted with a second nucleotide, which couples to the immobilized nucleotides in at least part of the first and at least part of the second predefined regions. By repeating these steps, an array of diverse oligonucleotides is formed on the substrate. The diverse oligonucleotides are then screened for specific binding to a receptor, e.g., a polynucleotide.

REFERENCES:
patent: 4562157 (1985-12-01), Lowe et al.
patent: 4689405 (1987-08-01), Frank et al.
patent: 4923901 (1990-05-01), Koester et al.
patent: 4973493 (1991-11-01), Guire
patent: 5079600 (1992-01-01), Schnur et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5405783 (1995-04-01), Pirrung et al.
Kleinfeld et al., "Controlled Outgrowth of Dissociated Neurons on Patterned Substrates," J. of Neuroscience, 8(11):4098-4120, Nov. 1988.
Haridasan et al., "Peptide Synthesis Using Photolytically Cleavable 2-Nitrobenzyloxycarbonyl Protecting Group," Proc. Indian Natl. Sci. Acad., Part A (1987) 53:717-728.
Sze/McGillis, VLSI Technology, Chapter 7, pp. 267-301, McGraw-Hill, 1983.
Geysen et al., "Strategies for epitope analysis using peptide synthesis," J. Immunol. Meth. (1987) 102:259-274.
Furka et al., "More Peptides by Less Labor," Abstract No. 288 from Xth International Symposium on Medicinal Chemistry, Budapest, Hungary, Aug. 15-19, 1988.
Ohtsuka et al., "Studies on transfer ribonucleic acids and related compounds. IX(1) Ribooligonucleotide synthesis . . . 2'-hydroxyl group," Nucleic Acids Research (1974) 1:1351-1357.
Hames et al., Nucleic Acid Hybridization: A Practical Approach (1985) (IRL Press Oxford England) pp. 126-128.

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