Synthesis and application of procainamide analogs for use in...

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

Reexamination Certificate

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C436S523000, C436S056000, C568S306000

Reexamination Certificate

active

07858399

ABSTRACT:
The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3:and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

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Ruo et al. Identificaiton of desethyl procainamide in patients: a new metabolite of procainamide. The Journal of Pharmacology and Experimental Therapeutics 1981, vol. 216, pp. 357-362.

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