Synergistic method for host cell transformation

Details Synergistic method for host cell transformation Synergistic method for host cell transformation

1989-09-28

1994-02-15

Benzion, Gary

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351722, 4352404, 4353171, C12N 1500

Patent

active

052866343

ABSTRACT:
This invention addresses the problem of inserting foreign or nonative DNA into a cell and, most particularly, into a plant cell. The solution to this problem of DNA transfer set forth in this invention is the use of a polycationic compound and cationic liposomes which act synergistically to accomplish host cell transformation.

REFERENCES:
Theodore M. Klein, et al., "Transfer of Foreign Genes into Intact Maize Cells with High-Velocity Microprojectiles", Proc. Natl. Acad. Sci. USA, 85, p. 4305 (1988).
Petrukhina, et al., "The Regulation of Liposome Permeability by Polyelectroylyte", J. Controlled Release, 3, (1986) 137-141.
Felgner et al. (1987) Proceeding Nat. Acad. Sci. vol. 84, pp. 7413-7417.
Petrukhina et al. (1986) J. Controlled Release vol. 3 (2-3) pp. 137-142.
Steinbliss et al. (1983) International Review of Cytology Supplement 16, pp. 191-208.

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