Synergistic antifungal protein and compositions containing same

Details Synergistic antifungal protein and compositions containing same Synergistic antifungal protein and compositions containing same

1995-06-01

1996-09-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 4352523, 514 2, 514 8, 514 12, 530372, 530376, 536 221, 536 231, 536 236, C12N 1500, C12N 120, C07H 1900, C07H 2100

Patent

active

055590349

ABSTRACT:
Novel plant proteins (SAFPs) which synergize the activity of antifungal antibiotics are identified. SAFPs are demonstrated to synergize antifungal antibiotics, such as nikkomycins, polyoxins and amphotericins. SAFPs alone also display antifungal activity against several species of fungi, including strains of Candida, Trichoderma, Neurospora and strains of the plant pathogens Fusarium, Rhizoctonia and Chaetomium. Synergistic antifungal compositions containing SAFP and antifungal antibiotics are provided. In particular, synergistic compositions of corn-SAFP (zeamatin), sorghum-SAFP (sormatin) or oat-SAFP (avematin) and nikkomycin are found to be effective as antifungal compositions, especially against the opportunistic human pathogen Candida albicans. Method for employing SAFPs and synergistic compositions containing them for the inhibition of fungi are provided. In addition, a method for purifying SAFP from grain meal is provided.

REFERENCES:
patent: 4486451 (1984-12-01), Linton et al.
European International Search Report dated Dec. 1, 1992.
European International Search Report dated May 29, 1995.
Brogmeyer, J. R., et al., "Isolation and Characterization of 25 kDa Antifungal Protein from Flax Seeds", Biochemical and Biophysical Research Communications, 187: 480-487 (1992).
Frendo, P., et al., "Abiotic stresses induce a thaumatin-like protein in maise: cDNA isolation and sequence analysis", Biological Abstracts, 94: AB-546 (1992).
Hejgaard, J., et al., "Two antifungal thaumatin-like proteins from barley grain", FEBS 10261, 291: 127-131 (1991).
Hejgaard, J., et al., "Antifungal activity of chitin-binding PR-4 type proteins from barley grain and stressed leaf", FEBS 11387, 307: 389-392 (1992).
Huynh, Q. K., et al., "Isolation and Characterization of A 22 kDa Protein with Antifungal Properties form Maize Seeds", Biochemical and Biophysical Research Communiations, 182: 1-5 (1992).
Leah, R., et al., "Biochemical and Molecular Characterization of Three Barley Seed Proteins with Antifungal Properties", The Journal of Biological Chemistry, 266: 1564-1573 (1991).
Logemann, L., et al., "Expression of a Barley Ribosome-Inactivating Protein Leads to Increased Fungal Protection in Transgenic Tobacco Plants", Biotechnology,10: 305-308 (1992).
Malehorn, D. E., et al., "Characterization and Expression of an Antifungal Zeamatin-like Protein (Zip) Gene from Zea mays", Plant Physiol. , 106: 1471-1481 (1994).
Postein, A. S., et al., "A Novel Pathogen--and Wound-Inducible Tobacco (Nicotiana tabacum) Protein with Antifungal Activity", Plant Physiol., 104: 109-118 (1994).
Richardson, M., et al., "A possible function for thaumtin and a TMV-induced protein suggested by homology to a maize inhibitor", Nature, 327: 432-434 (1987).
Roberts, W. K., et al.,"Zeamatin, an antifungal protein from maize with membrane-permeabilizing activity", Journal of General Microbiology, 136: 1771-1778 (1990).
Sambrook, J., et al., "Generation of Probes Specific for Uncloned Genes by Selective Amplification of Particular Segments of cDNA", Molecular Cloning, 2: 14.7-14.8 (1989).
Van Den Elzen, P. J. M., et al., "Virus and fungal resistance: from laboratory to field", Phil. Trans. R. Soc. Lond. B, 342: 271-278 (1993).
Vigers, A. J., et al., "A New Family of Plant Antifungal Proteins", Molecular Plant-Microbe Interations, 4: 315-323 (1991).
Vigers, A. J., et al., "Thaumatin-like pathogenesis-related proteins are antifungal", Plant Science, 83: 155-161 (1992).
Witty, M., "Preprothaumatin II is Processed to Biological Activity in Solanum Tuberosum", Biotechnology Letters, 12: 131-136 (1990).
Becker et al., Antimicro. Agents Chemother., 23: 926-929 (1983).
Bhakdi and Tranum-Jensen, Reviews of Physiology, Biochemistry and Pharmacology, 107: 147-223 (1984).
Blanco-Labra et al., J. Food Biochem., 5(1): 1-17 (1987).
Blanco-Labra et al., abstract only, J. Food Biochem. , 5(1): 1-17 (1981) (in Chem. Abstracts 96: 158/18m, p. 340, 1982).
Bohlmann et al., EMBO J., 7: 1559-1565 (1988).
Bryngelsson and Green, Physiol. and Mol. Plant Path., 35: 45-52 (1989).
Chalkley et al., Sabouraudia, 23: 147-164 (1985).
Coleman and Roberts, Biochim. Biophys. Acta, 659: 57-66 (1981).
Coleman and Roberts, Biochim. Biophys. Acta, 696: 239-244 (1982).
Cornelissen et al., Nature, 321: 531-532 (1986).
Data Sheet providing a comparison of the partial sequence of zeamatin with that of MAI as disclosed in Richardson et al., Nature, 327: 432-434 (1987).
Davies and Pope, Nature, 273: 235-236 (1978).
Edens et al., Gene, 18: 1-12 (1982).
Hare and Loebenberg, ASM News, 54(5): 235-239 (1988).
Hector and Braun, Antimicro. Agents Chemother., 29: 389-394 (1986).
Hector et cl., Antimicro. Agents Chemother., 34(4): 587-593 (1990).
McCarthy et al., J. Gen. Microbiol., 131: 775-780 (1985).
Mehta et al., Antimicro. Agents Chemother., 25: 373-374 (1984).
Pierpont et al., Physiol. Mol. Plant Pathol., 31: 291-298 (1987).
Pope amd Davies, Postgraduate Med. J. , 55: 674-676 (1979).
Roberts et al., Ann. N.Y. Acad. Sci., 544: 141-151 (1988).
Roberts and Selitrennikoff, Biochem. Biophys. Acta, 880: 161-170 (1986).
Roberts and Selitrennikoff, J. Gen. Microbiol., 134: 169-176 (1988).
Roberts and Stewart, Biochemistry, 12: 2615-2621 (1978).
Selitrennikoff, C. P., Biotechnology of Filamentous Fungi, "Screening for Antifungal Drugs", Chapter 8, pp. 189-217 (1992).
Sing et al., Plant Physiol., 85: 529-536 (987).
Van Den Elzen, P. J. M., et al., "Virus and fungal resistance: from laboratory to field", Phil. Trans. R. Soc. Lond. B, 342: 271-278 (1993).
Yadan et al., J. Bacteriol., 160: 884-88 (1984).
Yphantes et al., J. Bacteriol., 94: 1509-1515 (1967).

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