Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2007-03-20
2007-03-20
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S366000, C435S384000, C435S387000, C435S404000, C435S405000, C435S406000, C435S002000
Reexamination Certificate
active
10263955
ABSTRACT:
The present invention relates to a method for in vitro maturation of oocytes comprising the steps of:(a) culturing one or more GV oocytes in a culture medium, the culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors, the culturing taking place for a time period sufficient for cytoplasmatic maturation to occur;(b) washing the GV oocytes of step (a) to remove the nuclear maturation inhibiting substance;(c) culturing the washed oocytes of step (b) in a culture medium comprising one or more gonadotropins and/or one or more growth factors and/or MAS for a time period sufficient for nuclear maturation.The invention also relates to an oocyte culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors. The nuclear maturation inhibiting substance may be a MAS antagonist or an FF-MAS synthesis inhibitor, preferably a cytochrome P450 lanosterol 14α-demethylase (P45014DM) inhibitor e.g. ketoconazole or 22-hydroxycholesterol. The one or more gonadotropins and/or one or more growth factors are preferably a combination of EGF and FSH and/or LH.The invention also relates to the use of a nuclear maturation inhibiting substance and one or more gonadotropins and/or one or more growth factors as described above for the preparation of a cell culture medium for in vitro maturation of oocytes.
REFERENCES:
patent: 4987080 (1991-01-01), Grob et al.
patent: 6518262 (2003-02-01), Leysen et al.
patent: 2199663 (1998-09-01), None
patent: WO 99/61010 (1999-12-01), None
patent: WO 01/38493 (2001-05-01), None
Lu, Zhongxian, et al., Molecular and Cellular Endocrinology 164 (2000) 191-196.
Tsafriri, A., et al., Molecular Human Reproduction vol. 4, No. 5, pp. 483-489, 1998.
J. Smith et al., Human Reproduction, (1999), vol. 14, pp. 145-161.
Andersen Claus Yding
Byskov Anne Grete
Began Marc A.
Bork Richard W.
Green Reza
Lankford , Jr. Leon B.
Novo Nordisk A S
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